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保存条件 :-20℃
保质期 :半年
英文名 :pET303/CT-His
库存 :200
供应商 :沪震生物
规格 :5ug
pET303/CT-His
产品信息
产品货号 | 产品名称 | 产品规格 | 优惠价 |
---|---|---|---|
HZ0386 | pET303/CT-His | 20μl |
¥请询价 |
使用说明
沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。
基本信息
启动子: | T7/lac |
---|---|
复制子: | ColE1 ori,F1 ori |
终止子: | T7 terminator |
质粒分类: | 大肠杆菌载体;PET系列表达质粒 |
质粒大小: | 5369bp |
质粒标签: | C-6×His |
原核抗性: | 氨苄青霉素Amp |
克隆菌株: | DH5α |
培养条件: | 37℃,有氧,LB |
表达宿主: | BL21(DE3) |
诱导方式: | IPTG或乳糖及其类似物 |
5'测序引物: | T7:TAATACGACTCACTATAGGG |
3'测序引物: | T7-ter:TGCTAGTTATTGCTCAGCGG |
质粒简介
The Champion pET302/NT-His and pET303/CT-His Vector Kit is designed for cloning a gene of interest via restriction enzyme(s) and ligase (REaL) and subsequent high-level expression from the strong bacteriophage T7 promoter. In addition to the T7 promoter, each vector contains only the necessary functional elements and an N- or C-terminal 6xHis tag (pET302/NT-His and pET303/CT-His, respectively) for convenient purification and detection (Figure 1). Expression levels obtained from these vectors may be higher than those obtained from another suppliers pET vector (Figure 2). To maximize expression, use with MagicMedia E. coli Expression Medium.
质粒图谱
上海沪震实业有限公司
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入驻年限:11年