载体pET-29b

价格： ¥800 - 1380

品牌：HZbscience

货号：HZ0713

产品详情

pET-29b

产品信息

产品货号	产品名称	产品规格	优惠价
HZ0713	pET-29b	20μl	¥请询价

使用说明

沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。

基本信息

启动子:	T7/lac
复制子:	ColE1 ori，F1 ori
终止子:	T7 terminator
质粒分类:	大肠杆菌载体；PET系列表达质粒
质粒大小:	5370bp
质粒标签:	N-S, N-Thrombin, C-6×His
原核抗性:	卡那霉素Kan
克隆菌株:	DH5α
培养条件:	37℃，有氧，LB
表达宿主:	BL21(DE3)
诱导方式:	IPTG或乳糖及其类似物
5'测序引物:	T7:TAATACGACTCACTATAGGG
3'测序引物:	T7-ter:TGCTAGTTATTGCTCAGCGG

质粒简介

The pET-29a-c(+) vectors carry an N-terminal S•Tag™/thrombin configuration plus an optional C-terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer.