肿瘤坏死因子α(TNFa)检测试剂盒

价格： ¥2117

品牌：cloud-clone 品牌认证

货号：SEA133Mu

产品详情

ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)

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Product No.SEA133Mu
Organism SpeciesMus musculus (Mouse) Same name, Different species.
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test MethodDouble-antibody Sandwich
Assay Length3h
Detection Range15.6-1,000pg/mL
SensitivityThe minimum detectable dose of this kit is typically less than 6.1pg/mL.
SpecificityThis assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Alpha (TNFa).
No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.
ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
DownloadInstruction Manual
Format48T96T 96T*5 96T*10 96T*100
FOBUS$ 504
For negotiated price and more details, please contact local distributors!

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.