保存条件 ：-20摄氏度保存

保质期 ：1年

英文名 ：FUGW

库存 ：大量

供应商 ：上海沪震生物科技有限公司

规格 ：盒

FUGW产品信息

订购信息

货号	载体名称	出品公司	载体用途	价格	到货周期
HZ0367	FUGW		慢病毒载体	1000	现货

产品参数

Gene/insert name: flap-Ub promoter-GFP-WRE
Vector backbone: HR'CS-G
Growth strain(s): Stbl3

载体描述

Plasmid pFUGW was constructed by inserting the following into the multicloning site of HR'CS-G: HIV-1 flap sequence PCR-amplified
from the HIV NLA4.3 genome, the human polyubiquitin promoter-C (gift of L. Thiel, Amgen), the EGFP gene, and the WRE (woodchuck
hepatitis virus posttranscriptional regulatory element) (gift of D. Trono, University of Geneva). Lentiviruses can be produced by cotransfecting the HIV-1 packaging vector Delta8.9 and the VSVG envelope glycoprotein into 293 fibroblasts.

Order of elements: CMV LTR PstI flap PacI Ubiquitin promoter SpeI HindIII PstI SalI XbaI BamHI SmaI KpnI GFP NotI EagI XbaI EcoRI EcoRV HindIII ClaI WRE ClaI SalI XhoI KpnI 3'LTR ApaI PmeI.

载体序列

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质粒图谱