保存条件 ：-20ºC
保质期 ：2年
英文名 ：pSpark® DNA Cloning vectors
库存 ：20 rxn
供应商 ：Canvax
pSpark® I DNA Cloning Kit
For highly Efficient, Accurate & Robust general Cloning from PCR High Fidelity fragments, without toxic genes use
Product Overview:
pSpark® I is a highly efficient, accurate and easy-to-use DNA cloning System based on a novel breakthrough technology to generate blunt vectors with a high blunt cloning efficiency.
The vector is prepared by digestion of pSpark® at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.
- General cloning.
- Cloning of High Fidelity PCR amplified products.
- Production of ssDNA.
- Blue/white screening for recombinants.
- In vitro transcription from T7/SP6 dual-opposed promoters.
Advantages & Features
- Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
- Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding “suicide” strategies from toxic genes.
- Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation.
- High stability: eliminates cloning bias or pitfalls.
- Powerful: clone from less than 1 ng/kb, obtain 5-fold more positive colonies using 10x less DNA insert.
- Compatible with blue/white screening.
- Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
- Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
- Eliminates positive selection vector.
- High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed.
- Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation.
- Risk-free: product covered by our Quality 100% Guarantee.
Includes for 20 rxn:
– 20 µL pSpark® I (20 ng/µL)
– 20 µL T4 DNA Ligase (5U/Weiss)
– 200 µL T4 DNA Ligase Buffer (5x)
– 150 µL PEG 6000 (10x)
– 5 µL Insert Control 1 kb (20 ng/µL)
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
- Shelf Life: 24 months from the date of purchase, if it is properly stored.
pSpark® DNA Cloning vectors
Highest cloning Efficiency
and <1% Background.
From < 1 ng/kb.
Simplest PCR cloning process.
