Capan-1 人胰腺癌细胞/STR鉴定/镜像绮点(Cellverse)

Capan-1 人胰腺癌细胞/STR鉴定/镜像绮点(Cellverse)

价格: ¥1500

品牌:iCell(赛百慷) 品牌认证

货号:Capan-1

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英文名 :Capan1

库存 :100万

供应商 :镜像绮点

肿瘤类型 :胰腺癌

细胞类型 :细胞系

ATCC Number :100万

品系 :细胞系

组织来源 :胰腺

相关疾病 :胰腺癌

物种来源 :组织

免疫类型 :

细胞形态 :上皮细胞样

是否是肿瘤细胞 :胰腺癌

器官来源 :胰腺

运输方式 :冻存/复苏

年限 :长期

生长状态 :贴壁生长

规格 :1*10^6

Capan-1 人胰腺癌细胞/STR鉴定

细胞介绍

该细胞来源于一位40岁白人男性患者的肝转移。细胞表达粘液素,Rh+, HLA A2,,A9,B13,B17。含有刺激素受体和乙二醇激素受体。

细胞特性

1) 来源:胰腺癌,肝转移

2) 形态:上皮细胞样,贴壁生长

3) 含量:>1x106 个

4) 污染:支原体、细菌、酵母和真菌检测为阴性

5) 规格:T25瓶或者1mL冻存管包装

Capan-1 人胰腺癌细胞/STR鉴定

细胞培养步骤

一.培养基及培养冻存条件准备:

1) 准备IMDM(推荐iCell-0008)培养基;优质胎牛血清,20%;双抗1%。

Capan-1 细胞ATCC官网有关细胞的说明:

Capan-1 cells typically recover very slowly from cryopreservation, and may need more than a week before the cells attach well and grow sufficiently to be subcultured. Unfortunately this is normal for this cell line. It is normal for cultures of Capan-1 to have many cells which remain in suspension, these cells are viable and should be retained by gentle centrifugation and added back to the adherent population (in the same flask). Do not separate the populations (or discard floating cells) because the culture will become too dilute. Patience is the key to culturing these cells. HTB-79 cells grow as adherent patches with an epithelial-like morphology. Cells on the edges of a patch are somewhat elongated, while cells in the center of the patch are cuboidal and small. Growth will be patchy initially and the cells are very small with epithelial-like morphology. Theese cultures only become 80-90% confluent and will begin to detach at that confluence. There are also normally empty spaces in the culture vessels.

2) 培养条件: 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。

3) 冻存液:90%血清,10%DMSO,现用现配。

镜像绮点(上海)细胞技术有限公司

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入驻年限:10年

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地址:上海

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