CCDC101 Antibody (OADC00034)

抗体英文名 ：CCDC101 Antibody (OADC00034)

靶点 ：TRDMT1

应用范围 ：ELISA, WB

宿主 ：Rabbit

适应物种 ：Mouse

标记物 ：Unconjugated

克隆性 ：Polyclonal

保存条件 ：Store at -20C. Avoid repeated freeze/thaw cycles.

形态 ：Liquid. Whole antiserum from rabbit containing 0.05% azide.

规格 ：100 ul

NCBI Gene Id: 13434;
Swissprot Id: O14717
Dnmt2 (UniProtKB/Swiss-Prot entry O14717) belongs to the family of DNA cytosine methyltransferases. Dnmt2, however, lacks the large N-terminal regulatory domain common to other eukaryotic methyltransferases and does not methylate CpG in genomic DNA. Instead, it specifically methylates cytosine 38 in the anticodon loop of Asp transfer RNA. - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OADC00034

Mouse Dnmt2

Determination of the titer. To determine the titer, an ELISA was performed using a serial dilution of the Aviva antibody directed against mouse Dnmt2. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:21,000.

Mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a)

Western blot analysis using the Aviva antibody directed against Dnmt2 (1)r. Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Aviva antibody against mouse Dnmt2, diluted 1:1000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (47 kDa) is indicated on the right.