HDAC9 Antibody - N-terminal region (OAAB07338)

抗体英文名 ：HDAC9 Antibody - N-terminal region (OAAB07338)

靶点 ：HDAC9

应用范围 ：WB, IP, IF, IHC

宿主 ：Rabbit

适应物种 ：Human, Mouse

克隆性 ：Polyclonal

保存条件 ：Maintain refrigerated at 2-8C for up to 6 months. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.

形态 ：Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

亚型 ：Ig

规格 ：400ul

NCBI Gene Id: 9734
Swissprot Id: Q9UKV0
Purified Rabbit Polyclonal Antibody (Pab) - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OAAB07338

HDAC9

Figure 2: Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid. MITR is detected by using the HDAC9 N-term antibody (top panel) or a FLAG antibody (bottom panel) detecting a FLAG epitope fused at the N-term end of the MITR construct. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.

human cancer tissue

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

HeLa-HDAC9

This figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).

HeLa, HeLa-HDAC9 transfected cell

Both anti-HDAC9 N-term (OAAB07338) and C-term (AP1109b) Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone.

C2C12 myoblasts

Figure 1: Immunoblots for MITR (OAAB07338 HDAC9 N-term antibody), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts. Before cell collection for fractionation, the cells are transfected with plasmids coding for Mirk (Wt), kinase-inactive Mirk (YF) or MITR. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.