| Catalog Number: 21236 |
| Gene Symbol: SUMO1 |
| Description: Anti-SUMO-1 Rabbit polyclonal Antibody |
| Background: Small Ubiquitin-like Modifier or SUMO proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2 and -3 show a high degree of similarity to each other and are distinct from SUMO-1. |
| Immunogen: A synthetic peptide corresponding to residues near the C-terminal of human SUMO-1. |
| Applications: ELISA, WB, IHC, IP |
Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purified from serum |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Detects SUMO-1 but not SUMO-2/3 from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
Download Datasheet:  |
| Western blot: |
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Western blot analysis of over-expressed SUMO-1 proteins. HEK293 cells were transfected with SUMO-1 (lane 2) and SUMO-2 (lane 3) cDNAs and control empty vector (lane 1). Cell lysates were blotted with anti-SUMO-1 rabbit polyclonal antibody (Cat. # 21236). |
Publications:1. The SUMOylation of zinc-fingers and homeoboxes 1 (ZHX1) by Ubc9 regulates its stability and transcriptional repression activity
Journal of Cellular Biochemistry Volume 114, Issue 10, pages 2323–2333, October 20132. C-terminal Src Kinase (Csk)-mediated Phosphorylation of Eukaryotic Elongation Factor 2 (eEF2) Promotes Proteolytic Cleavage and Nuclear Translocation of eEF2
J Biol Chem. 2014 May 2;289(18):12666-78 |
