Anti-CTNNB1(S33F) Mouse Monoclonal Antibody点突变抗体

价格： ￥4800

品牌：纽斯特

货号：26304

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免疫原：A synthetic peptide from the internal region of CTNNB1 which includes the mutation of S33F, human origin.

亚型：IgG

形态：液体

保存条件：-20℃

克隆性：单克隆

标记物：FITC

适应物种：脊椎动物

保质期：两年

抗原来源：Mouse

目录编号：26304

级别：实验级

库存：30000

供应商：武汉纽斯特生物技术有限公司

宿主：Mouse

应用范围：ELISA, WB, IHC

浓度：1mg/ml

靶点：Beta-catenin, CTNNB, Catenin beta-1

抗体英文名：CTNNB1(S33F) antibody

抗体名：CTNNB1(S33F)抗体

规格：100μl

CTNNB1 (S33F) [26304]
[100ul]

Catalog Number: 26304

Gene Symbol: Beta-catenin, CTNNB, Catenin beta-1

Description: Anti-CTNNB1(S33F) Mouse Monoclonal Antibody

Background: CTNNB1 protein is a dual function protein. It is a subunit of a complex of proteins that from adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells. CTNNB1 protein also pulls double duty as an intracellular signal transducer in the Wnt signaling pathway. Mutations of CTNNB1 have been implicated in the pathogenesis of several cancers.

Immunogen: A synthetic peptide from the internal region of CTNNB1 which includes the mutation of S33F, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 2 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes S33F mutant, but not wild-type CTNNB1 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant CTNNB1 (S33F) and wildtype proteins. Purified His-tagged CTNNB1 (S33F) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti-CTNNB1 (S33F) monoclonal antibody (Cat. #26304).

Immunofluorescence:

Immunofluorescence of cells expressing CTNNB1 proteins with anti-CTNNB1 (S33F) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTNNB1 (WT) plasmid (left column) or pCDNA3-GFP-CTNNB1 (S33F) plasmid (right column), then fixed and stained with anti-CTNNB1 (S33F) monoclonal antibody (Cat. #26304).