Anti Ras (Q61L) Mouse Monoclonal Antibody点突变抗体

价格： ￥4800

品牌：N&T Biosciences

货号：26193

产品详情

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免疫原： A synthetic peptide from the internal region of Ras which includes the mutation of Q61L, human origin.

亚型：IgG

形态：液体

保存条件：-20℃

克隆性：单克隆

标记物：FITC

适应物种：脊椎动物

保质期：两年

抗原来源：Mouse

目录编号：26193

级别：实验级

库存：30000

供应商：NewEastBio

宿主：Mouse

应用范围：ELISA、WB、IF、IHC

浓度：1 mg/ml

靶点：RAS (Q61L)

抗体英文名：Ras(Q61L) Antibody

抗体名：Ras(Q61L)

规格：100μl

Ras (Q61L) [26193]
[100 µL]

Catalog Number: 26193

Gene Symbol: RAS (Q61L)

Description: Anti-Ras (Q61L) Mouse Monoclonal Antibody

Background: Ras (Q61L) is one type of Ras missence mutations at codon 61,Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation,and cell survival. Ras Q61L mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.

Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of Q61L, human origin.

Applications: ELISA, WB, IF, IHC

Recommended Dilutions:
ELISA 1:100-1:5000
WB 1:100-1:1000
IF 1:50-1:100
IHC 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes Ras (Q61L), but not wild type Ras protein from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant Ras proteins. Equal amount of purified Ras Q61H (lane 1), Ras Q61L (lane 2), Ras Q61R (lane 3), kRas WT (lane 4), hRas WT (lane 5), then were loaded and blotted with anti-Ras Q61L mouse monoclonal antibody (Cat. #26193).

Immunofluorescence:

Immunofluorescence of cells expressing Ras proteins with anti-Ras (Q61L) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras (Q61L) plasmid, pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (Q61H) plasmid, pCDNA3-GFP-Ras (Q61R) plasmid or pCDNA3-GFP-Ras (Q61K) plasmid, then fixed and stained with anti-Ras (Q61L) monoclonal antibody (Cat. #26193).

Publications:

1. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
Gene Therapy 21, 888-896 (October 2014)

2. Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136