Anti RAS (A146P) Mouse Monoclonal Antibody点突变抗体

Anti RAS (A146P) Mouse Monoclonal Antibody点突变抗体

价格: ¥4800

品牌:N&T Biosciences

货号:26478

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免疫原 : A synthetic peptide from the internal region of Ras which includes the mutation of A146P, human origin.

亚型 :IgG

形态 :液体

保存条件 :-20℃

克隆性 :单克隆

标记物 :FITC

适应物种 :脊椎动物

保质期 :两年

抗原来源 :Mouse

目录编号 :26478

级别 :实验级

库存 :30000

供应商 :NewEastBio

宿主 :Mouse

应用范围 :ELISA、IF、IHC

浓度 :1 mg/ml

靶点 :H-Ras、K-Ras、N-Ras

抗体英文名 :RAS(A146P) Antibody

抗体名 :RAS(A146P)

规格 :100μl

Ras (A146P) [26478]
[100ul]
Catalog Number: 26478
Gene Symbol: H-Ras; K-Ras; N-Ras
Description: Anti-RAS (A146P) Mouse Monoclonal Antibody
Background: The A146P mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146P mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of A146P, human origin.
Applications: ELISA, IF, IHC
Recommended Dilutions:
ELISA: 1:1000-1:5000
IF: 1:50-1:100
IHC: 1:50-1:100
Concentration: 1 mg/ml
Host: Mouse
Format: Liquid
Clonality: Monoclonal
Isotype:
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes A146P mutant, but not wild-type RAS of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Immunofluorescence:
Immunofluorescence of cells expressing KRas proteins with anti-KRas (A146P) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRas (A146P) plasmid , pCDNA3-GFP-KRas (WT) plasmid, pCDNA3-GFP-KRas (A146V) plasmid or pCDNA3-GFP-KRas (A146T) plasmid, then fixed and stained with anti-KRas (A146P) monoclonal antibody (Cat. #26478).
Publications:
1. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
Gene Therapy 21, 888-896 (October 2014)
2. Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136

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地址:武汉

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