Anti-RAS (G13V) Mouse Monoclonal Antibody点突变抗体

价格： ￥4800

品牌：N&T Biosciences

货号：26477

产品详情

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免疫原：A synthetic peptide from the internal region of Ras which includes the mutation of G13V, human origin.

亚型：IgG

形态：液体

保存条件：-20℃

克隆性：单克隆

标记物：FITC

适应物种：脊椎动物

保质期：两年

抗原来源：Mouse

目录编号：26477

级别：实验级

库存：30000

供应商：NewEastBio

宿主：Mouse

应用范围：ELISA、WB、IHC

浓度：1 mg/ml

靶点：H-Ras、K-Ras、N-Ras

抗体英文名：RAS(G13V) Antibody

抗体名：RAS(G13V)

规格：100μl

Ras (G13V) [26477]
[100ul]

Catalog Number: 26477

Gene Symbol: H-Ras; K-Ras; N-Ras

Description: Anti-RAS (G13V) Mouse Monoclonal Antibody

Background: Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13V mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.

Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of G13V, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes G13V mutant, but not wild-type RAS of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant Ras proteins. Purified His-tagged Ras (G13V) protein (lane 1), corresponding wildtype protein (lane 2), His-tagged Ras (G13S) protein (lane 3), His-tagged Ras (G13C) protein (lane 4), His-tagged Ras (G13R) protein (lane 5), His-tagged Ras (G13A) protein (lane 6) were blotted with anti-Ras (G13V) monoclonal antibody (Cat. #26477).

Immunofluorescence:

Immunofluorescence of cells expressing Ras proteins with anti-Ras (G13V) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras (G13V) plasmid ,pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (G13S) plasmid, pCDNA3-GFP-Ras (G13C) plasmid, pCDNA3-GFP-Ras (G13R) plasmid, pCDNA3-GFP-Ras (G13A) plasmid or pCDNA3-GFP-Ras (G13D) plasmid, then fixed and stained with anti-Ras (G13V) monoclonal antibody (Cat. #26477).

Publications:

1. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
Gene Therapy 21, 888-896 (October 2014)

2. Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136