小鼠Gsk3b过表达慢病毒转染实验

 

名称:
GSK3b; 糖原合成酶激酶 3β

 

描述:
Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. GSK3 has been implicated in fundamental cell processes such as cell fate determination,metabolism, transcriptional control and oncogenesis. Two isoforms, alpha (GSK3A) and beta, show a high degree of amino acid homology within their catalytic domains. GSK3B is involved in energy metabolism, neuronal cell development and body pattern formation。

糖元合成激酶3b(GSK3b)是一种参与了多种细胞活动的丝氨酸/色氨酸激酶. 其酪氨酸残基被磷酸化后表现出活化. Hartigan et al 首先证实GSK3b在体内和原位都是Pyk-2的作用底物. GSK3b的酪氨酸被磷酸化后表现出活性。Wnt信号途径中GSK3b的抑制导致β catenin的积累（β catenin在引导区中分布，参与星状胶质细胞迁移），而另外一个GSK的底物APC是极性形成必需的。

 

说明：
可提供一站式服务含病毒载体构建、病毒包装、细胞转染、建系（流式分选或者药物筛选）、PCR及WB验证、细胞功能实验、动物实验

 

参考文献:

# GSK3B polymorphisms alter transcription and splicing in Parkinson's disease Ann Neurol. 2005 Dec;58(6):829-39.

 

说明：
可提供一站式服务含病毒载体构建（3对引物）、外筛成功、病毒包装、细胞转染、建系（流式分选或者药物筛选）、PCR及WB验证、细胞功能实验、动物实验。