| Catalog Number: 26215 |
| Gene Symbol: PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha |
| Description: Anti-PIK3CA (D549N) Mouse Monoclonal Antibody |
| Background: Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer. |
| Immunogen: A synthetic peptide from the internal region of PIK3CA which includes the mutation of D549N, human origin. |
| Applications: ELISA, WB, IHC |
Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100 |
| Concentration: 0.5 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes D549N mutant, but not wild-type PIK3CA of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
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| Western blot: |
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Western blot analysis of recombinant PIK3CA (D549N) and wildtype proteins. Purified His-tagged PIK3CA (D549N) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-PIK3CA (D549N) monoclonal antibody (Cat. #26215). |
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Immunofluorescence of cells expressing PIK3CA proteins with anti-PIK3CA (D549N) antibody. HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA (D549N) plasmid (right column), then fixed and stained with anti-PIK3CA (D549N) monoclonal antibody (Cat. #26215). |
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