抗体名 ：SMAD4单克隆抗体
抗体英文名 ：SMAD4 Mouse Antibody
靶点 ：SMAD4, DPC4, JIP, MADH4, MYHRS
浓度 ：1mg/ml
应用范围 ：ELISA, WB, IHC
宿主 ：Mouse
适应物种 ：脊椎动物
保质期 ：2年
抗原来源 ：Mouse
目录编号 ：26453
级别 ：试验级
库存 ：30000
供应商 ：NewEastBio
标记物 ：FITC
克隆性 ：单克隆
保存条件 ：-20℃
形态 ：液体
亚型 ：IgG
免疫原 ：Purified SMAD4 protein, human origin. .
规格 ：100μl
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| Catalog Number: 26453 |
| Gene Symbol: SMAD4, DPC4, JIP, MADH4, MYHRS |
| Description: Anti-SMAD4 Mouse Monoclonal Antibody |
| Background: SMAD4 family member 4, also known as SMAD4, is a protein that in human is encoded by the SMAD4 gene. It is often found mutated in many cancers. It acts as a tumor suppressor that functions in the regulation of the TGF-? signal transduction pathway, which negatively regulates growth of epithelial cells and the extracellular matrix (ECM). SMAD4 alterations have been found in multiploid colorectal cancer and pancreatic carcinoma. It is found inactivated in at least 50% of pancreatic cancers. It is also found mutated in the autosomal dominantdisease juvenile polyposis syndrome (JPS). |
| Immunogen: Purified SMAD4 protein, human origin. . |
| Applications: ELISA, WB, IHC |
Recommended Dilutions:
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes SMAD4 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
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Western blot analysis of recombinant SMAD4 protein. Purified His-tagged SMAD4 protein (1-552aa, 65kd) and was blotted with anti-SMAD4 monoclonal antibody (Cat. #26453). |
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Immunofluorescence of cells expressing SMAD4 proteins with anti-SMAD4 antibody. HEK293T cells were transfected with pCDNA3-GFP-SMAD4 (WT) plasmid, then fixed and stained with anti-SMAD4 monoclonal antibody (Cat. #26453). |
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