Anti-NFE2L2(L30F) Mouse Monoclonal Antibody点突变抗体

价格： ￥4800

品牌：纽斯特

货号：26332

产品详情

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免疫原：A synthetic peptide from the internal region of NFE2L2 which includes the mutation of L30F, human origin.

亚型：IgG

形态：液体

保存条件：-20℃

克隆性：单克隆

标记物：FITC

适应物种：脊椎动物

保质期：两年

抗原来源：Mouse

目录编号：26332

级别：实验级

库存：30000

供应商：武汉纽斯特生物技术有限公司

宿主：Mouse

应用范围：ELISA、WB、IHC

浓度：1 mg/ml

靶点：NFE2L2、NRF2

抗体英文名：NFE2L2(L30F)

抗体名：NFE2L2(L30F)

规格：100μl

NFE2L2 (L30F) [26332]
[100ul]

Catalog Number: 26332

Gene Symbol: NFE2L2, NRF2

Description: Anti-NFE2L2 (L30F) Mouse Monoclonal Antibody

Background: NFE2L2 protein is encoded by NFE2L2 gene. It is a basic leucine zipper protein that regulates the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation. Several drugs that stimulate the NFE2L2 pathway are being studied for treatment of diseases that are caused by oxidative stress.

Immunogen: A synthetic peptide from the internal region of NFE2L2 which includes the mutation of L30F, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes L30F mutant, but not wild-type NFE2L2 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant NFE2L2 (L30F) and wildtype proteins. Purified His-tagged NFE2L2 (L30F) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti-NFE2L2 (L30F) monoclonal antibody (Cat. #26332).

Immunofluorescence:

Immunofluorescence of cells expressing NFE2L2 proteins with anti-NFE2L2 (L30F) antibody. HEK293T cells were transfected with pCDNA3-GFP-NFE2L2 (WT) plasmid (left column) or pCDNA3-GFP-NFE2L2 (L30F) plasmid (right column), then fixed and stained with anti-NFE2L2 (L30F) monoclonal antibody (Cat. #26332).