Anti-IDH2 (R172H) Mouse Monoclonal Antibody点突变

价格： ¥4800

品牌：N&T Biosciences

货号：26084

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免疫原：Mouse

亚型：IgG

形态：液体

保存条件：-20℃

克隆性：单克隆

标记物：FITC

适应物种：脊椎动物

保质期：两年

抗原来源：Mouse

目录编号：26084

级别：实验级

库存：30000

供应商：NewEastBio

宿主：Mouse

应用范围：ELISA、WB、IHC、IF

浓度：1 mg/ml

靶点：IDH2

抗体英文名：IDH2 (R172H) Antibody

抗体名：IDH2 (R172H)

规格：100μl

IDH2 (R172H) [26084]
[100 µL]

Catalog Number: 26084

Gene Symbol: IDH2

Description: Anti-IDH2 (R172H) Mouse Monoclonal Antibody

Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-172 in IDH2 to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

Immunogen: A synthetic peptide from the internal region of human IDH2 which includes the mutation of R172H.

Applications: ELISA, WB, IHC, IF

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100
IF: 1:50-1:100

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-IDH2(R172H) antibody recognizes IDH2(R172H) of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

	Western blot analysis of recombinant IDH2(R172H) and wild-type IDH2 proteins. Purified his-tagged IDH2(R172H) protein (Cat. # 10201) and wild-type IDH2 protein (Cat. # 10200) were blotted with anti-IDH2(R172H) monoclonal antibody (Cat. # 26084) (left panel). Proteins loading were blotted with anti-IDH2 monoclonal antibody (right panel).
Immunofluorescence:

Immunofluorescence of cells expressing IDH2 proteins with anti-IDH2 (R172H) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2 (R172H) plasmid (right column), then fixed and stained with anti-IDH2 (R172H) monoclonal antibody (Cat. #26084).

Publications:

1. Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11

2. Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
Nature 513, 110–114 (04 September 2014)

3. Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015