Anti-RAS (K117N) Mouse Monoclonal Antibody

价格： ￥4800

品牌：纽斯特

货号：26468

产品详情

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免疫原： A synthetic peptide from the internal region of Ras which includes the mutation of K117N, human origin.

亚型：IgG

形态：液体

保存条件：-20℃

克隆性：单克隆

标记物：FITC

适应物种：脊椎动物

保质期：2年

抗原来源：Mouse

目录编号：26468

级别：试验级

库存：3000000

供应商：武汉纽斯特生物技术有限公司

宿主：Mouse

应用范围： ELISA, WB, IHC

浓度：1mg/ml

靶点： H-Ras; K-Ras; N-Ras

抗体英文名：RAS(K117N) Antibody

抗体名：RAS(K117N)抗体

规格：100μl

Ras (K117N) [26468]
[100ul]

Catalog Number: 26468

Gene Symbol: H-Ras; K-Ras; N-Ras

Description: Anti-RAS (K117N) Mouse Monoclonal Antibody

Background: The K117N mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras K117N mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.

Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of K117N, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes K117N mutant, but not wild-type RAS of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant Ras (K117N) and wild-type proteins. Purified His-tagged Ras (K117N) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with anti-Ras (K117N) monoclonal antibody (Cat. #26468).

Immunofluorescence:

Immunofluorescence of cells expressing KRas proteins with anti-KRas (K117N) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRas (WT) plasmid (left column) or pCDNA3-GFP-KRas (K117N) plasmid (right column), then fixed and stained with anti-KRas (K117N) monoclonal antibody (Cat. #26468).

Publications:

1. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
Gene Therapy 21, 888-896 (October 2014)

2. Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136