Anti-EGFR (A839T) Mouse Monoclonal Antibody点突变

抗体名 ：抗EGFR (A839T) 鼠单克隆抗体

抗体英文名 ： Anti-EGFR (A839T) Mouse Monoclonal Antibody

靶点 ：EGFR/HER1/ERBB1

浓度 ：1mg/ml

应用范围 ：ELISA, WB, IF, IHC

宿主 ：鼠

适应物种 ：脊椎动物

保质期 ：2年

目录编号 ：26117

级别 ：实验级

库存 ：300

供应商 ：NewEastbio

克隆性 ：单克隆

保存条件 ： -20°C

形态 ：液体

免疫原 ：A synthetic peptide from the internal region of EGFR which includes the mutation of A839T, human origin.

规格 ：100μl

EGFR (A839T) [26117] [100 µL] Catalog Number: 26117 Gene Symbol: EGFR/HER1/ERBB1 Description: Anti-EGFR (A839T) Mouse Monoclonal Antibody Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of A839T, human origin. Tested Applications: ELISA, WB, IF, IHC Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 Concentration: 1 mg/ml Host: Mouse Format: Liquid Clonality: Monoclonal Isotype: Purity: Purified from ascites Storage buffer: Preservative: no Constituents: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity: Recognizes EGFR (A839T), but not wild type EGFR of vertebrates. Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles. Western blot: Western blot analysis of recombinant EGFR(A839T) and wildtype proteins. Purified His-tagged EGFR (A839T) protein (702-987) (Cat. #10221) and wild-type protein (702-987) (Cat. #10210) were blotted with anti- EGFR (A839T) mouse antibody (Cat. #26117). Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (A839T) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (A839T) plasmid (right column), then fixed and stained with anti- EGFR (A839T) monoclonal antibody (Cat. #26117). Publications: 1. Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma Tumor Biology. October 2014