Anti-IDH1 (R132S) Mouse Monoclonal Antibody点突变抗体

价格： ￥4800

品牌：纽斯特

货号：26160

产品详情

相关推荐

免疫原：Mouse

亚型：IgG

形态：液体

保存条件：-20°C

克隆性：单克隆

标记物：FITC

适应物种：脊椎动物

保质期：两年

抗原来源：Mouse

目录编号：26160

级别：实验级

库存：30000

供应商：武汉纽斯特生物技术有限公司

宿主：Mouse

应用范围：ELISA、WB、IHC

浓度：1 mg/ml

靶点：IDH1/IDCD/IDH/IDP/IDPC/PICD

抗体英文名：IDH1 (R132S) Antibody

抗体名：IDH1 (R132S)

规格：100μl

IDH1 (R132S) [26160]
[100 µL]

Catalog Number: 26160

Gene Symbol: IDH1; IDCD; IDH; IDP; IDPC; PICD

Description: Anti-IDH1 (R132S) Mouse Monoclonal Antibody

Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

Immunogen: A synthetic peptide from the internal region of IDH1 which includes the mutation of R132S, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes IDH1 (R132S) mutated prteein, but not wild-type IDH1 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant IDH1 (R132S) and wild-type proteins. Purified His-tagged IDH1 (R132S) protein (lane 2) and wild-type protein (lane 1) were blotted with anti-IDH1 (R132S) mouse antibody (Cat. #26160).

Immunofluorescence:

Immunofluorescence of cells expressing IDH1 proteins with anti-IDH1 (R132S) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH1 (WT) plasmid (left column) or pCDNA3-GFP-IDH1 (R132S) plasmid (right column), then fixed and stained with anti-IDH1 (R132S) monoclonal antibody (Cat. #26160).

Publications:

1. Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11

2. Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
Nature 513, 110–114 (04 September 2014)

3. Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015