Anti-IDH1 (R132H) Mouse Monoclonal Antibody点突变抗体

价格： ￥4800

品牌：纽斯特

货号：26081

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免疫原：Mouse

亚型： IgG

形态：液体

保存条件：-20°C.

克隆性：单克隆

标记物：FITC

适应物种：脊椎动物

保质期：两年

抗原来源：Mouse

目录编号：26081

级别：实验级

库存：30000

供应商：武汉纽斯特生物技术有限公司

宿主：Mouse

应用范围：ELISA、WB、IHC、IP

浓度：1 mg/ml

靶点：IDH1

抗体英文名：IDH1 (R132H) Antibody

抗体名：IDH1 (R132H)

规格：100μl

IDH1 (R132H) [26081]
[100 µL]

Catalog Number: 26081

Gene Symbol: IDH1

Description: Anti-IDH1 (R132H) Mouse Monoclonal Antibody

Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

Immunogen: Recombinant protein of human IDH1 which includes the mutation of R132H.

Applications: ELISA, WB, IHC, IP

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg ²⁺and Ca ²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-IDH1(R132H) antibody recognizes IDH1(R132H) of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

	Western blot analysis of recombinant IDH1(R132H) and wild-type proteins. Purified His-tagged IDH1 (R132H) protein (Cat.#10199) and wild-type protein (amino acid 1-240) (Cat.#10198) were blotted with anti-IDH1(R132H) mouse antibody (Cat.# 26081)(Left panel) or with anti-IDH1 mouse antibody (Cat. #26136 right panel).
Immunofluorescence:
	Immunofluorescence of cells expressing IDH1 proteins with anti-IDH1(R132H) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH1 (WT) plasmid (left column) or pCDNA3-GFP-IDH1 (R132H) plasmid (right column), then fixed and stained with anti-IDH1 (R132H) monoclonal antibody (Cat. #26081).