Anti-MEK1 (P264S) Mouse Monoclonal Antibody

价格： ￥4800

品牌：纽斯特生物

货号：26266

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免疫原：Mouse

亚型：IgG

形态：液体

保存条件：-20°C

克隆性：单克隆

标记物：FITC

保质期：两年

抗原来源：Mouse

目录编号：26266

级别：实验级

库存：3000

供应商：武汉纽斯特生物技术有限公司

宿主：Mouse

应用范围：ELISA、WB、IHC

浓度：0.3 mg/ml

靶点：MAP2K1/MAPKK1/MEK1/MKK1/PRKMK1

抗体英文名：MEK1 (P264S) Antibody

抗体名：MEK1 (P264S)

规格：100μl

MEK1 (P264S) [26266]
[100ul]

Catalog Number: 26266

Gene Symbol: MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1

Description: Anti-MEK1 (P264S) Mouse Monoclonal Antibody

Background: MEK1 (also known as extracellular signal-regulated kinases, ERKs) plays important role in the MAP kinase cascade, which transduces multiple extracellular signals to control cell growth, proliferation and differentiation. Activated upon growth factors stimulation, MEK1 phosphorylates MAPK3/ERK1 and MAPK1/ERK2, thus activates the MAP pathway and regulates transcription. Abnormal of the MEK1 protein, including point mutations, are implicated in diseases such as cardiofaciocutaneous syndrome (CFC) and melanoma.

Immunogen: A synthetic peptide from the internal region of MEK1 which includes the mutation of P264S, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 0.3 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes P264S mutant, but not wild-type MEK1 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant MEK1 (P264S) and wildtype proteins. Purified His-tagged MEK1 (P264S) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-MEK1 (P264S) monoclonal antibody (Cat. #26266).

Immunofluorescence:

Immunofluorescence of cells expressing MEK1 proteins with anti-MEK1 (P264S) antibody. HEK293T cells were transfected with pCDNA3-GFP-MEK1 (WT) plasmid (left column) or pCDNA3-GFP-MEK1 (P264S) plasmid (right column), then fixed and stained with anti-MEK1 (P264S) monoclonal antibody (Cat. #26266).