抗体名 ：ATM 抗体
抗体英文名 ：ATM antibody
靶点 ：ATM
浓度 ：1mg/ml
应用范围 ：ELISA, WB, IHC
宿主 ：Mouse
适应物种 ：脊椎动物
保质期 ：两年
抗原来源 ：Rabbit
目录编号 ：21276
级别 ：实验级
库存 ：30000
供应商 ：武汉纽斯特生物技术有限公司
标记物 ：FITC
克隆性 ：单克隆
保存条件 ：-20℃
形态 ：液体
亚型 ：IgG
免疫原 ： Purified ATM protein, human origin.
规格 ：100μl
| Catalog Number: 21276 |
| Gene Symbol: ATM |
| Description: Anti-ATM Rabbit Polyclonal Antibody |
| Background: Ataxia telangiectasia mutated (ATM) is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. It phosphorylates several key proteins that initiate activation of the DNA damage checkpoint, leading to cell cycle arrest, DNA repair or apoptosis. Activity of ATM protein is under tight control, and mutation of ATM can cause disease such as Ataxia telangiectasia (AT) and cancers. |
| Immunogen: Purified ATM protein, human origin. |
| Applications: ELISA, WB, IHC |
Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200 |
| Concentration: 0.2mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purified from serum |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes ATM of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
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| Western blot: |
 |
Western blot analysis of recombinant ATM protein. Purified His-tagged ATM protein was blotted with anti-ATM rabbit polyclonal antibody (Cat. #21276). |
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Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti-ATM polyclonal antibody (Cat. #21276). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody. |
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