抗体名 ：ALK抗体
抗体英文名 ：ALK antibody
靶点 ：ALK, CD246, NBLST3
浓度 ：1mg/ml
应用范围 ：ELISA, WB, IHC
宿主 ：Mouse
适应物种 ：脊椎动物
保质期 ：两年
抗原来源 ：Mouse
目录编号 ：21280
级别 ：实验级
库存 ：30000
供应商 ：NewEastBio
标记物 ：FITC
克隆性 ：单克隆
保存条件 ：-20℃
形态 ：液体
亚型 ：IgG
免疫原 ： Purified ALK protein, human origin.
规格 ：100μl
| Catalog Number: 21280 |
| Gene Symbol: ALK, CD246, NBLST3 |
| Description: Anti-ALK Rabbit Polyclonal Antibody |
| Background: Anaplastic lymphoma kinase (ALK) also known as ALK tyrosine kinase receptor or CD246 (cluster of differentiation 246) is a neuronal orphan receptor tyrosine kinase. ALK is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Abnormal ALK, by fusion with other genes, gaining additional gene copies or mutations, can be oncogenic. |
| Immunogen: Purified ALK protein, human origin. |
| Applications: ELISA, WB, IHC |
Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200 |
| Concentration: 0.2mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purified from serum |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes ALK of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
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| Western blot: |
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Western blot analysis of recombinant ALK protein. Purified His-tagged ALK protein was blotted with anti-ALK rabbit polyclonal antibody (Cat. #21280). |
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Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti-ALK polyclonal antibody (Cat. #21280). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-Rabbit IgG (1:50 dilution) was used as secondary antibody. |
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