抗体名 ：AKT1抗体
抗体英文名 ：AKT1 antibody
靶点 ： AKT1, AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
浓度 ：1mg/ml
应用范围 ：ELISA, WB, IHC
宿主 ：Mouse
适应物种 ：脊椎动物
保质期 ：两年
抗原来源 ：Mouse
目录编号 ：21274
级别 ：实验级
库存 ：30000
供应商 ：NewEastBio
标记物 ：FITC
克隆性 ：单克隆
保存条件 ：-20℃
形态 ：液体
亚型 ：IgG
免疫原 ： Purified AKT1 protein, human origin.
规格 ：100μl
| Catalog Number: 21274 |
| Gene Symbol: AKT1, AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA |
| Description: Anti-AKT1 Rabbit Polyclonal Antibody |
| Background: AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Its function is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. There are evidences that mutation or abnormal expression in AKT1 is associated with cancers. |
| Immunogen: Purified AKT1 protein, human origin. |
| Applications: ELISA, WB, IHC |
Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200 |
| Concentration: 0.2mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purified from serum |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes AKT1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
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| Western blot: |
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Western blot analysis of recombinant AKT1 protein. Purified His-tagged AKT1 protein was blotted with anti-AKT1 rabbit polyclonal antibody (Cat. #21274). |
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Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti-AKT1 polyclonal antibody (Cat. #21274). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody. |
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