Anti-β-catenin Rabbit Polyclonal Antibody

价格： ￥1600

品牌：纽斯特生物

货号：21282

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抗体名：β-catenin

抗体英文名：β-catenin Rabbit Polyclonal Antibody

靶点：CTNNB1, CTNNB

浓度：1 mg/ml

应用范围： ELISA, WB, IF, IHC

宿主：Rabbit

保质期：一年

抗原来源：Rabbit

目录编号：21282

级别：实验级

库存：30000

供应商：武汉纽斯特生物技术有限公司

标记物：FITC

克隆性：单克隆

保存条件：-20℃

形态：液体

亚型：IgG

免疫原：A synthetic peptide from the internal region of β-catenin

规格：100μl

β-catenin [21282]
[100 µL]

Catalog Number: 21282

Gene Symbol: CTNNB1, CTNNB

Description: Anti-β-catenin Rabbit Polyclonal Antibody

Background: Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.

Immunogen: A synthetic peptide from the internal region of β-catenin.

Applications: ELISA, WB, IF, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:2000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Rabbit

Format: Liquid

Clonality: Polyclonal

Isotype:

Purity: Purified from serum

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes β-catenin from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant β-catenin protein. Purified His-tagged β-catenin protein (amino acids 1-76) was blotted with anti- β-catenin rabbit polyclonal antibody (Cat. #21282).

Immunohistochemistry:

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-β-catenin polyclonal antibody (Cat. #21282). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.