Anti-Vimentin Mouse Monoclonal Antibody

Anti-Vimentin Mouse Monoclonal Antibody

价格: ¥2600

品牌:纽斯特

货号:26054.

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免疫原 :Mouse

亚型 :IgG

形态 :液体

保存条件 : -20°C

克隆性 :单克隆

标记物 :FITC

适应物种 :脊椎动物

保质期 :两年

抗原来源 :Mouse

目录编号 :26054

级别 :实验级

库存 :30000

供应商 :武汉纽斯特生物技术有限公司

宿主 :Mouse

应用范围 :ELISA、WB、IHC、IP

浓度 :1 mg/ml

靶点 :Vimentin

抗体英文名 :Vimentin Antibody

抗体名 :Vimentin

规格 :100μl

Vimentin [26054]
[100 µL]
Catalog Number: 26054
Gene Symbol: VIM
Description: Anti-Vimentin Mouse Monoclonal Antibody
Background: Vimentin is an intermediate filament protein found in various non-epithelial cells, especially in mesenchymal cells. It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.
Immunogen: A synthetic peptide from the internal region of human vimentin protein.
Applications: ELISA, WB, IHC, IP
Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100
Concentration: 1 mg/ml
Host: mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-vimentin antibody recognizes vimentin of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Western blot:
Western blot analysis of recombinant vimentin protein. Purified His-tagged vimentin protein (Cat.#10190) was blotted with anti-vimentin monoclonal antibody (Cat.# 26054).

Immunohistochemical analysis of paraffin-embedded human renal cancer tissue

with anti-vimentin monoclonal antibody (Cat. #26091). Tissue samples were fixed

with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval
was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary
antibody (1:100 dilution) overnight at 4°C.A HRP-conjugated Goat anti-mouse IgG (1:50 dilution)
was used as secondary antibody

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入驻年限:8年

联系人:黄先生

地址:武汉

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