Catalog Number: 26036 |
Gene Symbol: RAS (G12D) |
Description: Anti-Ras(G12D) Mouse Monoclonal Antibody |
Background: Ras (G12D) is one type of Ras missense mutations at the codon 12. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G12D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers. |
Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of G12D, human origin. |
Applications: ELISA, WB, IF, IHC |
Recommended Dilutions: ELISA: 1:100-1:3000 WB: 1:200-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host: mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG2a |
Purity: Purified from ascites |
Storage buffer: |
Preservative: no |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Ras(G12D) antibody recognizes Ras(G12D) proteins of vertebrates. |
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
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Western blot: |
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Western blot analysis of recombinant Ras WT and G12D proteins. Equal amounts of purified Ras WT (lane 1, Cat # 10172) and G12D (lane 2, Cat # 10154) proteins were loaded and blotted with anti-Ras G12D mouse monoclonal antibody (Cat # 26036) (Top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti-Ras antibody (Cat # 21021). |
Immunofluorescence of cells expressing RAS proteins with anti-RAS (G12D) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(G12D) plasmid, pCDNA3-GFP-Ras(WT)plasmid,pCDNA3-GFP-Ras(G12S) plasmid, pCDNA3-GFP-Ras(G12V)plasmid,pCDNA3-GFP-Ras(G12C)plasmid, pCDNA3-GFP-Ras(G12R)plasmid,or pCDNA3-GFP-Ras(G12A)plasmid, then fixed and stained with anti-Ras(G12D) monoclonal antibody (Cat. # 26036).
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