| Catalog Number: 21017 |
| Gene Symbol: RHOA |
| Description: Anti-RhoA Rabbit Polyclonal Antibody |
| Background: Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca2+ sensitivity of smooth muscle contraction. |
| Immunogen: A synthetic peptide from the C-terminal region of RhoA. |
| Applications: ELISA, WB, IHC, IP |
Recommended Dilutions:
ELISA: 1:100-1:5000
WB: 1:100-1:2000
IHC: 1:10-1:1000 |
| Concentration: 0.2 mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Purity: Purified from serum |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 51% glycerol Species |
| Species Reactivity: RhoA antibody recognizes RhoA from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
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| Western blot: |
 |
Western blot analysis of RhoA proteins. Purified recombinant His-RhoA protein was used for western blot with the anti-RhoA rabbit polyclonal antibody (Cat. # 21017). |
| Publications: |
1. Ethanol increases p190RhoGAP activity, leading to actin cytoskeleton rearrangements
J Neurochem. 2011 Dec;119(6):1306-16 |
2. The transcriptional coactivators megakaryoblastic leukemia 1/2 mediate the effects of loss of the tumor suppressor deleted in liver cancer 1
Oncogene. 2012 Aug 30;31(35):3913-23 |
3. G(alpha)12/13 inhibition enhances the anticancer effect of bortezomib through PSMB5 downregulation
Carcinogenesis. 2010 Jul;31(7):1230-7 |
4. Transgenesis of the Wolffian duct visualizes dynamic behavior of cells undergoing tubulogenesis in vivo
Development, Growth & Differentiation. Volume 55, Issue 4, pages 579–590, May 2013 |
5. Foxc2 induces Wnt4 and Bmp4 expression during muscle regeneration and osteogenesis
Cell Death and Differentiation (2013) 20, 1031–1042 |
6. N-glycosylation induces collagen triple helix repeat containing 1 (CTHRC1) and drives oral cancer cell migration
J Biol Chem. 2013 Jul 12;288(28):20217-27 |
7. Cyclodextrin protects podocytes in diabetic kidney disease
Diabetes. 2013 Nov;62(11):3817-27 |
8. Amyloid beta1-42 (Aβ42) up-regulates the expression of sortilin via the p75NTR/RhoA signaling pathway
Journal of Neurochemistry Volume 127, Issue 2, pages 152–162, October 2013 |
9. Podocyte-specific GLUT4 deficient mice have fewer and larger podocytes and are protected from diabetic nephropathy
Diabetes. 2014 Feb;63(2):701-14 |
10. Loss of TAK1 increases cell traction force in a ROS-dependent manner to drive epithelial–mesenchymal transition of cancer cells
Cell Death and Disease (2013) 4, e848 |
11. Syndecan-2 regulation of morphology in breast carcinoma cells is dependent on RhoGTPases
Biochimica et Biophysica Acta (BBA) - General Subjects Volume 1840, Issue 8, August 2014, Pages 2482–2490 |
12. Epithelial repair is a two-stage process driven first by dying cells and then by their neighbours
J Cell Sci. 2014 Mar 15;127(Pt 6):1229-41 |
13. MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells
Cytokine Volume 69, Issue 1, September 2014, Pages 47–55 |
14. Triclosan Potentiates Epithelial-To-Mesenchymal Transition in Anoikis-Resistant Human Lung Cancer Cells
PLoS One. 2014 Oct 16;9(10):e110851 |
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