| Catalog Number: 26005 |
| Gene Symbol: RAC1 |
| Description: Anti-Rac1 Mouse Monoclonal Antibody |
| Background: Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. In mammals, there are three Rac genes: Rac1, Rac2 and Rac3. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). |
| Immunogen: Recombinant full length protein of Rac1 |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA 1:1000-1:5000 WB 1:1000-1:2000 |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Rac1 antibody recognizes Rac1 from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of Rac1 proteins. MEF whole-cell lysate was used for western blots. The monoclonal anti-Rac1 antibody was pre-incubated without (lane 1) or with (lane 2) the antigen protein (Rac1) to show the specificity. |
| Publications: |
1. Phosphorylation of α-tubulin by protein kinase C stimulates microtubule dynamics in human breast cells
Cytoskeleton Volume 71, Issue 4, pages 257–272, April 2014 |
2. Immunofluorescence and Confocal Microscopy of Neutrophils
Neutrophil Methods and Protocols Methods in Molecular Biology Volume 1124, 2014, pp 251-268 |
3. A link between the nuclear-localized srGAP3 and the SWI/SNF chromatin remodeler Brg1
Molecular and Cellular Neuroscience Volume 60, May 2014, Pages 10–25 |
4. SHP2 Phosphatase Promotes Mast Cell Chemotaxis toward Stem Cell Factor via Enhancing Activation of the Lyn/Vav/Rac Signaling Axis
The Journal of Immunology April 14, 2014 1301155 |
5. ROBO1, a tumor suppressor and critical molecular barrier for localized tumor cells to acquire invasive phenotype: Study in African-American and Caucasian prostate cancer models
International Journal of Cancer Volume 135, Issue 11, pages 2493–2506, 1 December 2014 |
6. Transcription factors NRF2 and nf-kb are coordinated effectors of the RHO family, GTP binding protein rac1 during inflammation
J Biol Chem. 2014 May 30;289(22):15244-58 |
7. Canonical and Non-canonical G-protein Signaling Helps Coordinate Actin Dynamics to Promote Macrophage Phagocytosis of Zymosan
Mol Cell Biol. 2014 Nov 15;34(22):4186-99 |
8. p53 Down Regulates PDGF-Induced Formation of Circular Dorsal Ruffles in Rat Aortic Smooth Muscle Cells
PLoS One. 2014 Sep 23;9(9):e108257 |
9. LPA-mediated migration of ovarian cancer cells involves translocalization of Gαi2 to invadopodia and association with Src and β-pix
Cancer Lett. 2015 Jan 28;356(2 Pt B):382-91 |
10. cAMP controls the restoration of endothelial barrier function after thrombin-induced hyperpermeability via Rac1 activation
Physiological ReportsPublished 24 October 2014Vol. 2no. e12175DOI: 10.14814/phy2.12175 |
11. Cisplatin at Sub-toxic Levels Mediates Integrin Switch in Lung Cancer Cells
Anticancer Research December 2014 vol. 34 no. 12 7111-7117 |
12. Semaphorin-3F suppresses the stemness of colorectal cancer cells by inactivating Rac1
Cancer letters. March 1, 2015Volume 358, Issue 1, Pages 76–84. |
13. Geometry sensing through POR1 regulates Rac1 activity controlling early osteoblast differentiation in response to nanofiber diameter
Integr Biol (Camb). 2014 Dec 24 |
14. Inhibition of Rac1 Activity in the Hippocampus Impairs the Forgetting of Contextual Fear Memory
Mol Neurobiol. 2015 Jan 24 |
|
