Anti-Active Rab7 Mouse Monoclonal Antibody活性抗体

价格： ￥4800

品牌：纽斯特生物（New EastBiosciences)

货号：26923

产品详情

相关推荐

免疫原：Mouse

亚型：IgG

形态：液体

保存条件：-20℃

克隆性：单克隆

标记物：FITC

适应物种：脊椎动物

保质期：两年

抗原来源：Mouse

目录编号：26923

级别：实验级

库存：30000

供应商：武汉纽斯特生物技术有限公司

宿主：Mouse

应用范围：IP、IHC、IF

浓度：1mg/ml

靶点：Rab7

抗体英文名：Rab7-GTP antibody

抗体名：Rab7

规格：30μl

Active Rab7-GTP [NL26923]
[30 µL]

Catalog Number: NL26923

Gene Symbol: RAB7

Description: Anti-Active Rab7 Mouse Monoclonal Antibody

Background: Rab7 belongs to the Rab family of small GTPases. Rab7 is located in the late endosome, lysosome, cytoplasmic vesicle, phagosome and melanosome. It is involved in the late endocytic transport and contributes to the maturation of phagosomes.

Immunogen: Recombinant full length protein of active Rab7

Applications: IP, IHC, IF

Recommended Dilutions:
1 µg for ~2 mg total cellular proteins for IP
1:100 dilution for IHC, IF

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Rab7 antibody recognizes active Rab7 proteins of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:

Rab7 activation assay. Purified full-length Rab7 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab7 monoclonal antibody (Cat. No. 26923). Immunoblot was with an anti-Rab7 polyclonal antibody (Cat. No. 21069).

Publications:

1. VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors
Hepatology Volume 61, Issue 1, pages 361–374, January 2015

2. Rab5 Activity Regulates GLUT4 Sorting into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance
Endocrinology. 2014 Sep;155(9):3315-28