Anti-Active Rap1 Mouse Monoclonal Antibody活性抗体

价格： ￥4800

品牌：纽斯特生物（New EastBiosciences)

货号：26912

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免疫原：Mouse

亚型：IgG

形态：液体

保存条件：-20℃

克隆性：单克隆

标记物：FITC

适应物种：脊椎动物

保质期：两年

抗原来源：Mouse

目录编号：26912

级别：实验级

库存：30000

供应商：武汉纽斯特生物技术有限公司

宿主：Mouse

应用范围： IP、IHC

浓度：1mg/ml

靶点：Rap1

抗体英文名：Rap1-GTP antibody

抗体名：Rap1

规格：30μl

Active Rap1-GTP [NL26912]
[30 µL]

Catalog Number: NL 26912

Gene Symbol: RAP1

Description: Anti-Active Rap1 Mouse Monoclonal Antibody

Background: Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway, Raf-1 and B-Raf. Rap1 regulates cell adhesion mechanisms through effector molecules such as the integrins and cadherins.

Immunogen: Recombinant full length protein of active Rap1.

Applications: IP, IHC

Recommended Dilutions: 1 μg for 1~2 mg total cellular proteins for IP
1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Rap1 antibody recognizes active Rap1 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:

Rap1 activation assay. Purified Rap1 proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rap1 monoclonal antibody (Cat. No. 26912). Immunoblot was with an anti-Rap1 polyclonal antibody (Cat. No. 21104).

Publications:

1. Rap1 GTPase Activation and Barrier Enhancement in RPE Inhibits Choroidal Neovascularization In Vivo
PLoS One. 2013 Sep 10;8(9):e73070