Anti-active Rheb Mouse Monoclonal Antibody活性抗体

价格： ￥4800

品牌：纽斯特生物（New EastBiosciences)

货号：26910

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免疫原：Mouse

亚型：IgG

形态：液体

保存条件：-20℃

克隆性：单克隆

标记物：FITC

适应物种：Mouse

保质期：两年

抗原来源：Mouse

目录编号：26910

级别：实验级

库存：30000

供应商：武汉纽斯特生物技术有限公司

宿主：Mouse

应用范围： IP、 IHC

浓度：1mg/ml

靶点：Rheb

抗体英文名：Rheb-GTP antibody

抗体名：Rheb

规格：30μl

Active Rheb-GTP [NL26910]
[30 µL]

Catalog Number: NL26910

Gene Symbol: RHEB

Description: Anti-Active Rheb Mouse Monoclonal Antibody

Background: Small GTPases are a super-family of cellular signaling regulators. Rheb is a member of the Ras-superfamily GTPases. Rheb has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related (ATR).

Immunogen: Recombinant full length protein of active Rheb.

Applications: IP, IHC

Recommended Dilutions: 1 μg for 1~2 mg total cellular proteins for IP
1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgM

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Rheb antibody recognizes active Rheb of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Immunoprecipitation/Western blot:

Rheb activation assay. Purified Rheb proteins were immunoprecipitated after treated with GDP (lane 1) or GTP_γS (lane 2). Immunoprecipitation was done with the anti-active Rheb monoclonal antibody (Cat. No. 26910). Immunoblot was with an anti-Rheb rabbit polyclonal antibody (Cat. No. 21098).

Publications:

1. Inducible Nitric Oxide Synthase Drives mTOR Pathway Activation and Proliferation of Human Melanoma by Reversible Nitrosylation of TSC2
Cancer Res. 2014 Feb 15;74(4):1067-78

2. SynGAP Regulates Protein Synthesis and Homeostatic Synaptic Plasticity in Developing Cortical Networks
PLoS One. 2013 Dec 31;8(12):e83941