Anti active Ga13 Mouse Monoclonal Antibody活性抗体

价格： ￥4800

品牌：纽斯特生物（New EastBiosciences)

货号：26902

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免疫原：Mouse

亚型：IgG

形态：液体

保存条件：-20℃

克隆性：单克隆

适应物种：脊椎动物

保质期：两年

抗原来源：Mouse

目录编号：26902

级别：实验级

库存：30000

供应商：武汉纽斯特生物技术有限公司

宿主：Mouse

应用范围： IP、IHC

浓度：1mg/ml

靶点：Ga13

抗体英文名：Ga13-GTP antibody

抗体名：Ga13

规格：30ul

Active Gα13-GTP [NL26902]
[30 µL]

$495.00

Catalog Number: NL26902

Gene Symbol: Gna13

Description: Anti-Active Gα₁₃-GTP Mouse Monoclonal Antibody

Background: Heterotrimeric G proteins are essential cellular signal transducers. Gα₁₃is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα₁₃ are being explored.

Immunogen: Recombinant full length active Gα₁₃ protein

Applications: IP, IHC

Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP;
1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG2b

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Gα₁₃ monoclonal antibody recognizes active Gα₁₃ of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:

	IP/WB analysis of active Gα₁₃ proteins. Purified Gα₁₃ proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with anti-active Gα₁₃-GTP mouse monoclonal antibody (Cat. # 26902), these proteins were separated by SDS/PAGE and western blotted with anti- Gα₁₃ mouse monoclonal antibody (Cat. # 26004).
Immunohistochemistry:

Staining of MEF cells (treated with PDGF) with anti- Gα₁₃-GTP monoclonal antibody showing the localization of Gα₁₃-GTP in the leading edge of migrating cells.

Publications:

1. Resistance to inhibitors of cholinesterase-8A (Ric-8A) is critical for growth factor receptor-induced actin cytoskeletal reorganization
J Biol Chem. 2011 Sep 2;286(35):31055-61

2. Agonist activation of the G protein-coupled receptor GPR35 involves transmembrane domain III and is transduced via Galpha and beta-arrestin-2
Br J Pharmacol. 2011 Feb;162(3):733-48

3. Gα12/13 inhibition enhances the anti-cancer effect of bortezomib through PSMB5 downregulation
Carcinogenesis (2010)

4. Suppression of tumor angiogenesis by Galpha(13) haploinsufficiency
J Biol Chem. 2009 Oct 2;284(40):27409-15