anti-NSF

免疫原 ：KLH conjugated synthetic peptide derived from human NSF

亚型 ：IgG

形态 ：Lyophilized or Liquid

保存条件 ：Store at -20 °C for one year.

克隆性 ：单克隆

标记物 ：无

适应物种 ：Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep,

保质期 ：1年

目录编号 ：bs-11255R

库存 ：999

供应商 ：北京博奥森生物技术有限公司

宿主 ：Rabbit

应用范围 ：WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,ICC=1:100-500,

浓度 ：1mg/ml

抗体英文名 ：NSF

抗体名 ：N-乙基顺丁烯二酰亚胺敏感融合蛋白抗体

Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).

Function:
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.

Subunit:
Homohexamer. Interacts with GABARAP and GABARAPL2. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity (By similarity). Interacts with CDK16 (By similarity).

Subcellular Location:
Cytoplasm.

Post-translational modifications:
Phosphorylation at Ser-569 interferes with homohexamerization (By similarity).

Similarity:
Belongs to the AAA ATPase family.

SWISS:
P46459

Gene ID:
4905

Database links:

Entrez Gene: 4905 Human

Entrez Gene: 18195 Mouse

Entrez Gene: 60355 Rat

SwissProt: P46459 Human

SwissProt: P46460 Mouse

SwissProt: Q9QUL6 Rat



Sample:
U937 (Human) Cell Lysate at 30 ug
Spleen (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-NSF (bs-11255R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 83 kD
Observed band size: 83 kD




Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Cerebellum tissue lysates
Lane 5: Human SH-SY5Y cell lysates
Lane 6: Human HeLa cell lysates
Lane 7: Human THP-1 cell lysates
Primary: Anti-NSF (bs-11255R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 75 kDa