anti-Integrin beta 1

anti-Integrin beta 1

价格: 询价

品牌:Bioss 品牌认证

货号:bs-0486R

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免疫原 :KLH conjugated synthetic peptide derived from human Integrin beta 1

亚型 :IgG

形态 :Lyophilized or Liquid

保存条件 :Store at -20 °C for one year.

克隆性 :单克隆

标记物 :

适应物种 :Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,

保质期 :1年

目录编号 :bs-0486R

库存 :999

供应商 :北京博奥森生物技术有限公司

宿主 :Rabbit

应用范围 :Elisa=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,Flow Cyt=1μg/Test,IF=1:100-500,

浓度 :1mg/ml

抗体英文名 :Integrin beta 1

抗体名 :整合素β1/Integrin β1抗体

产品图片


Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: U-87MG (Human) Cell Lysate at 30 ug
Lane 3: Ear (Rat) Lysate at 40 ug
Lane 4: Ear (Mouse) Lysate at 40 ug
Lane 5: Bladder (Mouse) Lysate at 40 ug
Primary: Anti-Integrin beta 1 (bs-0486R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD






Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin beta 1) Polyclonal Antibody, Unconjugated (bs-0486R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.





Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin beta 1) Polyclonal Antibody, Unconjugated (bs-0486R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.





Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin beta 1) Polyclonal Antibody, Unconjugated (bs-0486R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.





Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin beta 1) Polyclonal Antibody, Unconjugated (bs-0486R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.





Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti-Integrin beta 1 antibody (bs-0486R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.





Blank control (Black line):HUVEC(Black).
Primary Antibody (green line): Rabbit Anti-Integrin beta 1 antibody (bs-0486R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.





Blank control: HUVEC cells(blue).
Primary Antibody:Rabbit Anti-CD29 antibody(bs-0486R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) .Primary antibody (bs-0486R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

已发表文献

[IF=5.168]Wang et al. Loss of calponin h1 confers anoikis resistance and tumor progression in the development of high-grade serous carcinoma originating from the fallopian tube epithelium. (2017) Oncotarget. 8:61133-61145WB, Human
[IF=3.58]Lei, Mingxing, et al. "Modulating hair follicle size with Wnt10b/DKK1 during hair regeneration." Experimental Dermatology (2014).IHC-P, Mouse
[IF=3.55]Fu, Min, et al. "Inositol Hexaphosphate and Inositol Inhibit Colorectal Cancer Metastasis to the Liver in BALB/c Mice." Nutrients 8.5 (2016): 286.IHC-P, Mouse
[IF=3.27]Oralova, V., et al. "Osteogenic Potential of the Transcription Factor c-MYB." Calcified Tissue International: 1-12.WB, Mouse
[IF=2.53]Ji, Kaiyuan, et al. "MicroRNA 143‐5p regulates alpaca melanocyte migration, proliferation, and melanogenesis." Experimental dermatology (2017).WB, Other Species
[IF=2.24]Chen, Xiaodong, et al. "The effect of porcine ADM to improve the burn wound healing." Int J Clin Exp Pathol 6.11 (2013): 2280-2291.WB, Rat
[IF=2.09]Qin, Jie, et al. "Concentrated growth factor promotes Schwann cell migration partly through the integrin β1-mediated activation of the focal adhesion kinase pathway." International Journal of Molecular Medicine.WB, Rat
[IF=2.02]Zhao, Xue, et al. "Knockdown of Snail inhibits epithelial-mesenchymal transition of human laryngeal squamous cell carcinoma Hep-2 cells through VDR signaling pathway." Biochemistry and Cell Biology ja (2017).WB, Human
[IF=1.89]Deng, Wei, et al. "Bone marrow mesenchymal stromal cells with CD47 high expression via the signal transducer and activators of transcription signaling pathway preventing myocardial fibrosis." International Journal of Clinical and Experimental Pathology 8.9 (2015): 10555-10564.other, Rat
[IF=1.41]Lu T et al. In vitro culture and biological properties of broiler adipose‑derived stem cells.Exp Ther Med. 2018 Sep;16(3):2399-2407.ICF&FITC, broiler

北京博奥森生物技术有限公司

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入驻年限:8年

联系人:销售部

地址:北京

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