anti-VEGFR2

免疫原 ：KLH conjugated synthetic peptide derived from human VEGFR2

亚型 ：IgG

形态 ：Lyophilized or Liquid

保存条件 ：Store at -20 °C for one year.

克隆性 ：单克隆

标记物 ：无

适应物种 ：Human,Mouse,Rat,Dog,Pig,Cow,

保质期 ：1年

目录编号 ：bs-0565R

库存 ：999

供应商 ：北京博奥森生物技术有限公司

宿主 ：Rabbit

应用范围 ：WB=1:500-2000,Elisa=1:500-1000,Flow Cyt=1μg/Test,

浓度 ：1mg/ml

抗体英文名 ：VEGFR2

抗体名 ：血管内皮生长因子受体2抗体

产品图片

Sample: Skin(Mouse) Lysate at 40 ug Primary: Anti-VEGFR2 (bs-0545R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 147 kD Observed band size: 147kD Sample: Embryon (Mouse)Lysate at 40 ug Primary: Anti-VEGFR2 (bs-0545R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 147 kD Observed band size: 147kD Tissue/cell: Rat liver ; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-VEGFR2/FLK1 Polyclonal , Unconjugated(bs-0565R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-VEGFR2 Polyclonal Antibody, FITC conjugated (bs-0565R-FITC), at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Tissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-VEGFR2 Polyclonal Antibody, PE conjugated (bs-0565R-PE), at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Tissue/cell: Human esophageal cancer tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-VEGFR2 Polyclonal Antibody, Unconjugated(bs-0565R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei 已发表文献 [IF=4.15]Wang, Nan, et al. "Vascular endothelial growth factor stimulates endothelial differentiation from mesenchymal stem cells via Rho/myocardin-related transcription factor-A signaling pathway." The International Journal of Biochemistry & Cell Biology (2013).WB, Human, Rat [IF=3.923]Li H et al. siRNA‐mediated silencing of PAI‐1 gene acts as a promoter over the recanalization of endothelial progenitor cells in rats with venous thrombosis. J Cell Physiol. 2019 Apr 14. ICC, Rat [IF=3.73]Gao, Jin-Hang, et al. "Celecoxib Ameliorates Portal Hypertension of the Cirrhotic Rats through the Dual Inhibitory Effects on the Intrahepatic Fibrosis and Angiogenesis." PLOS ONE 8.7 (2013): e69309.WB, IHC-P, Rat [IF=3.17]Li, Junqin, et al. "Fatty Acid Synthase Mediates the Epithelial-Mesenchymal Transition of Breast Cancer Cells." International Journal of Biological Sciences 10.2 (2014): 171-180.WB, Human [IF=2.84]Wei, Shuping, et al. "Targeted Contrast-Enhanced Ultrasound Imaging of Angiogenesis in an Orthotopic Mouse Tumor Model of Renal Carcinoma."Ultrasound in Medicine & Biology (2014)IHC-P, Mouse [IF=2.705]Liu Z et al. MRI analysis of hydrogel-loaded apatinib for local therapy of hepatocellular carcinoma model in nude mice. Biochem Biophys Res Commun. 2019 Feb 5;509(2):529-534. IHC, Human [IF=2.02]Wang, Zheng, et al. "RhGH attenuates ischemia injury of intrahepatic bile ducts relating to liver transplantation." Journal of Surgical Research 171.1 (2011): 300-310.IHC-P, Rat [IF=1.77]Wang et al. Effects of minocycline on apoptosis and angiogenesis-related protein expression in a rat model of intracerebral hemorrhage. (2012) Neural.Regen.Res. 7:595-600IHC-F, Rat [IF=1.069]Yang, Lei, et al. "Promotive effect of salvia extract on angiogenesis of myocardium in rats with myocardial infarction." Int J Clin Exp Med 10.6 (2017): 9245-9251.IHC-F, Rat [IF=0.78]Yang K et al. Expression and distribution of HIF-1α, HIF-2α, VEGF, VEGFR-2 and HIMF in the kidneys of Tibetan sheep, Plain sheep and goat. Folia Morphol (Warsz). 2020 Feb 5.IHC-P, Sheep [IF=0]Wan et al. Endostatin, an angiogenesis inhibitor, ameliorates bleomycin-induced pulmonary fibrosis in rats. (2013) Respir.Re. 14:56IHC-P, Rat