促销中..anti-Caspase-3

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研选促销中..anti-Caspase-3

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免疫原 :KLH conjugated synthetic peptide derived from human caspase-3 p17 subunit

亚型 :IgG

形态 :Lyophilized or Liquid

保存条件 :Store at -20 °C for one year.

克隆性 :多克隆

标记物 :

适应物种 :Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,

保质期 :1年

目录编号 :bs-0081R

库存 :999

供应商 :北京博奥森生物技术有限公司

宿主 :Rabbit

应用范围 :WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,Flow Cyt=2μg/Test,IF=1:100-500,

浓度 :1mg/ml

抗体英文名 :Caspase-3

抗体名 :活化半胱胺酸蛋白酶蛋白-3抗体



Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Lung (Mouse) Lysate at 40 ug
Lane 3: Lymph node (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Mouse) Lysate at 40 ug
Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 6: Spleen (Rat) Lysate at 40 ug
Lane 7: Lung (Rat) Lysate at 40 ug
Lane 8: Lymph node (Rat) Lysate at 40 ug
Lane 9: Cerebrum (Rat) Lysate at 40 ug
Lane 10: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-Caspase-3 (bs-0081R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 37 kD





Sample:
Lane 1: Raji (Human) Cell Lysate at 30 ug
Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 3: Lung (Mouse) Lysate at 40 ug
Lane 4: Lung (Rat) Lysate at 40 ug
Primary: Anti-Caspase-3 (bs-0081R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 37 kD






Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Caspase-3 (bs-0081R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 17 kD





Sample:
Lung(Mouse) Lysate at 40 ug
Hela(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-Caspase-3 (bs-0081R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35/29/19/17 kD
Observed band size: 38 kD






Tissue/cell: rat brain tissue; 4% PA-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Caspase-3 Polyclonal Antibody, Unconjugated(bs-0081R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining





Tissue/cell: rabbit pancreas tissue; 4% PA-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Caspase-3 Polyclonal Antibody, Unconjugated(bs-0081R) 1:300, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining



Tissue/cell: NIH/3T3 cell; 4% PA-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Caspase-3) polyclonal Antibody, Unconjugated (bs-0081R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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北京博奥森生物技术有限公司

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入驻年限:8年

联系人:销售部

地址:北京

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