研选促销中..anti-beta-Actin (Loading Control)

免疫原 ：Synthetic MAP peptide derived from human beta-Actin

亚型 ：IgG

形态 ：Lyophilized or Liquid

保存条件 ：Store at -20 °C for one year.

克隆性 ：多克隆

标记物 ：无

适应物种 ：Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Rabbit,Sheep,Bee,Fish,Guinea Pig,Hamster,Goat,Cat,mt,op

保质期 ：1年

目录编号 ：bs-0061R

库存 ：999

供应商 ：北京博奥森生物技术有限公司

宿主 ：Rabbit

应用范围 ：WB=1:5000-20000,Elisa=1:5000-20000,IHC-P=1:500-1000,Flow Cyt=1μg/Test,

浓度 ：1mg/ml

抗体英文名 ：beta-Actin (Loading Control)

抗体名 ：β-肌动蛋白/β-Actin 抗体（内参抗体）

产品图片

Sample: Lung (Mouse) Lysate at 40 ug Uterus (Mouse) Lysate at 40 ug Small intestine (Mouse) Lysate at 40 ug Primary: Anti- beta-Actin (bs-0061R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: A549 Cell (Human) Lysate at 30 ug Primary: Lane1: Anti-beta-Actin (bs-0061R) at 1/500 dilution Lane2: Anti-beta-Actin (bs-0061R) at 1/1000 dilution Lane3: Anti-beta-Actin (bs-0061R) at 1/2000 dilution Lane4: Anti-beta-Actin (bs-0061R) at 1/4000 dilution Lane5: Anti-beta-Actin (bs-0061R) at 1/5000 dilution Lane6: Anti-beta-Actin (bs-0061R) at 1/8000 dilution Lane7: Anti-beta-Actin (bs-0061R) at 1/10000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: A549 Cell (Human) Lysate at 30 ug Primary: Lane1: Anti-beta-Actin (bs-0061R) at 1/500 dilution Lane2: Anti-beta-Actin (bs-0061R) at 1/1000 dilution Lane3: Anti-beta-Actin (bs-0061R) at 1/2000 dilution Lane4: Anti-beta-Actin (bs-0061R) at 1/4000 dilution Lane5: Anti-beta-Actin (bs-0061R) at 1/5000 dilution Lane6: Anti-beta-Actin (bs-0061R) at 1/8000 dilution Lane7: Anti-beta-Actin (bs-0061R) at 1/10000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: Lane1: 293T Cell Lysate at 25 ug Lane2: A549 Cell Lysate at 25 ug Lane3: A431 Cell Lysate at 25 ug Primary: Anti- beta-Actin (bs-0061R) at 1/1000 and 1/5000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42kD Observed band size: 42 kD Sample: Lung lysate at 30ug; Primary: Anti-beta-actin (bs-0061R) at 1:1000 dilution Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1:3000 dilution Predicted band size : 42kD Observed band size : 42kD Sample: SH-SY5Y (Human) Lysate at 40 ug Primary: Lane 1: Anti-beta-Actin (bs-0061R) at 1/2000 dilution Lane 2: Anti-beta-Actin (bs-0061R) at 1/5000 dilution Lane 3: Anti-beta-Actin (bs-0061R) at 1/10000 dilution Lane 4: Anti-beta-Actin (bs-0061R) at 1/20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: Lymph node (Rat) Lysate at 40 ug Primary: Lane 1: Anti-beta-Actin (bs-0061R) at 1/1000 dilution Lane 2: Anti-beta-Actin (bs-0061R) at 1/2000 dilution Lane 3: Anti-beta-Actin (bs-0061R) at 1/5000 dilution Lane 4: Anti-beta-Actin (bs-0061R) at 1/10000 dilution Lane 5: Anti-beta-Actin (bs-0061R) at 1/20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: Thymus (Mouse) Lysate at 40 ug Primary: Lane 1: Anti-beta-Actin (bs-0061R) at 1/1000 dilution Lane 2: Anti-beta-Actin (bs-0061R) at 1/2000 dilution Lane 3: Anti-beta-Actin (bs-0061R) at 1/5000 dilution Lane 4: Anti-beta-Actin (bs-0061R) at 1/10000 dilution Lane 5: Anti-beta-Actin (bs-0061R) at 1/20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: HL60 (Human) Cell Lysate at 40 ug HUVEC (Human) Cell Lysate at 40 ug Spinal cord (Rat) Lysate at 40 ug Rectum (Rat) Lysate at 40 ug Placenta (Rat) Lysate at 40 ug Lymph node (Rat) Lysate at 40 ug Lung (Rat) Lysate at 40 ug Spleen (Rat) Lysate at 40 ug JAR (Human) Cell Lysate at 40 ug 293T (Human) Cell Lysate at 40 ug Jurkat (Human) Cell Lysate at 40 ug TM4 (Human) Cell Lysate at 40 ug Primary: Anti-beta-Actin (bs-0061R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: A549 (Human) Cell Lysate at 40 ug Raw264.7 (Mouse) Cell Lysate at 40 ug SH-SY5Y (Human) Cell Lysate at 40 ug MKN45 (Human) Cell Lysate at 40 ug CHO (Human) Cell Lysate at 40 ug Panc-1 (Human) Cell Lysate at 40 ug 4T1 (Human) Cell Lysate at 40 ug ASPC-1 (Human) Cell Lysate at 40 ug H9C2 (Rat) Cell Lysate at 40 ug Brl-3a (Rat) Cell Lysate at 40 ug TT (Human) Cell Lysate at 40 ug TEV-1 (Human) Cell Lysate at 40 ug EC9706 (Human) Cell Lysate at 40 ug Primary: Anti-beta-Actin (bs-0061R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: Embryo Cerebrum (Mouse) Lysate at 40 ug Du145 (Human) Lysate at 40 ug SW480 (Human) Cell Lysate at 40 ug U87MG (Human) Lysate at 40 ug U251 (Human) Lysate at 40 ug A673 (Human) Lysate at 40 ug Lovo (Human) Lysate at 40 ug 293FT (Human) Lysate at 40 ug JEG-3 (Human) Lysate at 40 ug RSC96 (Rat) Cell Lysate at 40 ug MCF-7 (Human) Cell Lysate at 40 ug HepG2 (Human) Lysate at 40 ug A431 (Human) Lysate at 40 ug Primary: Anti-beta-Actin (bs-0061R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: Thymus (Mouse) Lysate at 40 ug Urinary bladder (Mouse) Lysate at 40 ug Uterus (Mouse) Cell Lysate at 40 ug Aorta (Mouse) Lysate at 40 ug olfactory bulb (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Adrenal gland (Mouse) Lysate at 40 ug Ovary (Mouse) Lysate at 40 ug Ear (Mouse) Lysate at 40 ug U2os (Human) Cell Lysate at 40 ug ASPC-1 (Human) Cell Lysate at 40 ug Vas deferens (Mouse) Lysate at 40 ug trachea (Mouse) Lysate at 40 ug Primary: Anti-beta-Actin (bs-0061R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: Lung (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-beta-Actin (Loading Control) (bs-0061R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Tissue/cell: human cervical carcinoma; 4% Para.formaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Beta-actin Polyclonal Antibody, Unconjugated(bs-0061R) 1:1500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Hela cell; 4% Para.formaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. MCF7 cell; 4% Para.formaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. MCF7 cell; 4% Para.formaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control: NIH/3T3. Primary Antibody (green line): Rabbit Anti-beta-Actin (Loading Control) antibody (bs-0061R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-beta-Actin (Loading Control) antibody (bs-0061R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: RSC96(blue). Primary Antibody: Rabbit Anti-beta-Actin /FITC Conjugated antibody (bs-0061R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions. Protocol The cells were fixed with 2% para.formaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-0061R/FITC, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed. 更多产品信息可点击Bioss官网查看http://www.bioss.com.cn/