GRP78蛋白

英文名 ：GRP78 (Bip) Protein

供应商 ：研卉生物

规格 ：50ug

GRP78蛋白 GRP78 (Bip) Protein

Catalog No. SPR-107
Product Name

	GRP78 (Bip) Protein
Description	Active Human Recombinant GRP78 (Bip) Protein
Applications	WB , SDS-PAGE , ATPase Activity Assay , Functional Assay , ELISA
Concentration	Lot/batch specific. See included datasheet.
Conjugates	His tag
Nature	Recombinant
Species	Human
Expression System	E. coli
Amino Acid Sequence	MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL
Purity	>90%
Biological Activity	ATPase active

Storage Buffer

	20mM Tris, 50mM KCl, 5mM MgCl2, 1mM DTT, pH7.5
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Affinity Purified
Specificity	~78 kDa
Cite This Product	Human Recombinant GRP78 (Bip) Protein (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-107)
Certificate of Analysis	This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 2.3µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay.Alternative Names BIP Protein, Grp78 Protein, HSPA5 Protein, MIF2 Protein, immunoglobulin heavy chain binding Protein Research Areas Cancer, Heat Shock, Cell Signaling, Chaperones, Organelle Markers, Trafficking Cellular Localization Endoplasmic reticulum lumen, Melanosome Accession Number NM_005347 Gene ID 3309 Swiss Prot P11021 Scientific Background GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia (Barnes), to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7). References 1. Cho S., et al (2007) Mol Cell Biol. 27(1): 368-83. 2. Yang Y., et al. (1998) J Biol Chem. 273: 25552-25555. 3. Luo S., et al (2006) 26(15): 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19: 4333-4336. 6. Laquerre S., et al. (1998) J. Virology. 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res. 65(13): 5785-91. Product Images