pFL46S

保存条件 ：低温运输，-20℃保存

保质期 ：1年

英文名 ：pFL46S

库存 ：大量

供应商 ：信裕生物

名称	编号	规格	价格	手册
pFL46S

质粒编号:
质粒名称: pFL46S
其他名称:
质粒大小（bp）: 6078
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号: X70270
其他属性: pFL46S was constructed from pUC19 plasmid where two alu I sites were modified. The site 629 was replaced by a BglII linker and the site 747 by a ClaI site. The yeast selectable marker has been cloned in the BglII site and the 2 micron 0.5 Kb FspI fragment containing its replication origin has been cloned at the ClaI site. The order of the major features in this plasmid is:EcoRI/MCS/HindIII – LEU2 – 2 micron ori – pMB1 ori – ampR. YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 15.5. Restriction digests of the clone give the following sizes (kb): BamHI–6.1; BglII–3.3, 2.8; ClaI–3.7, 1.9, 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 397135 Hosts: E.coli HB101, S.cerevisiae, E.coli, Saccharomyces. Related vectors: pUC19, yeast 2-micron plasmid.
质粒图谱:

质粒序列:

1	tcgcgcgttt	cggtgatgac	ggtgaaaacc	tctgacacat	gcagctcccg	50
51	gagacggtca	cagcttgtct	gtaagcggat	gccgggagca	gacaagcccg	100
101	tcagggcgcg	tcagcgggtg	ttggcgggtg	tcggggctgg	cttaactatg	150
151	cggcatcaga	gcagattgta	ctgagagtgc	accatatgcg	gtgtgaaata	200
201	ccgcacagat	gcgtaaggag	aaaataccgc	atcaggcgcc	attcgccatt	250
251	caggctgcgc	aactgttggg	aagggcgatc	ggtgcgggcc	tcttcgctat	300
301	tacgccagct	ggcgaaaggg	ggatgtgctg	caaggcgatt	aagttgggta	350
351	acgccagggt	tttcccagtc	acgacgttgt	aaaacgacgg	ccagtgaatt	400
401	cgagctcggt	acccggggat	cctctagagt	cgacctgcag	gcatgcaagc	450
451	ttggcgtaat	catggtcata	gctgtttcct	gtgtgaaatt	gttatccgct	500
501	cacaattcca	cacaacatac	gagccggaag	cataaagtgt	aaagcctggg	550
551	gtgcctaatg	agtgagctaa	ctcacattaa	ttgcgttgcg	ctcactgccc	600
601	gctttccagt	cgggaaacct	gtcgtgccag	cagatctatt	acattatggg	650
651	tggtatgttg	gaataaaaat	caactatcat	ctactaacta	gtatttacgt	700
701	tactagtata	ttatcatata	cggtgttaga	agatgacgca	aatgatgaga	750
751	aatagtcatc	taaattagtg	gaagctgaaa	cgcaaggatt	gataatgtaa	800
801	taggatcaat	gaatattaac	atataaaatg	atgataataa	tatttataga	850
851	attgtgtaga	attgcagatt	cccttttatg	gattcctaaa	tcctcgagga	900
901	gaacttctag	tatatctaca	tacctaatat	tattgcctta	ttaaaaatgg	950
951	aatcccaaca	attacatcaa	aatccacatt	ctcttcaaaa	tcaattgtcc	1000
1001	tgtacttcct	tgttcatgtg	tgttcaaaaa	cgttatattt	ataggataat	1050
1051	tatactctat	ttctcaacaa	gtaattggtt	gtttggccga	gcggtctaag	1100
1101	gcgcctgatt	caagaaatat	cttgaccgca	gttaactgtg	ggaatactca	1150
1151	ggtatcgtaa	gatgcaagag	ttcgaatctc	ttagcaacca	ttattttttt	1200
1201	cctcaacata	acgagaacac	acaggggcgc	tatcgcacag	aatcaaattc	1250
1251	gatgactgga	aattttttgt	taatttcaga	ggtcgcctga	cgcatatacc	1300
1301	tttttcaact	gaaaaattgg	gagaaaaagg	aaaggtgaga	gccgcggaac	1350
1351	cggcttttca	tatagaatag	agaagcgttc	atgactaaat	gcttgcatca	1400
1401	caatacttga	agttgacaat	attatttaag	gacctattgt	tttttccaat	1450
1451	aggtggttag	caatcgtctt	actttctaac	ttttcttacc	ttttacattt	1500
1501	cagcaatata	tatatatata	tttcaaggat	ataccattct	aatgtctgcc	1550
1551	cctaagaaga	tcgtcgtttt	gccaggtgac	cacgttggtc	aagaaatcac	1600
1601	agccgaagcc	attaaggttc	ttaaagctat	ttctgatgtt	cgttccaatg	1650
1651	tcaagttcga	tttcgaaaat	catttaattg	gtggtgctgc	tatcgatgct	1700
1701	acaggtgtcc	cacttccaga	tgaggcgctg	gaagcctcca	agaaggttga	1750
1751	tgccgttttg	ttaggtgctg	tgggtggtcc	taaatggggt	accggtagtg	1800
1801	ttagacctga	acaaggttta	ctaaaaatcc	gtaaagaact	tcaattgtac	1850
1851	gccaacttaa	gaccatgtaa	ctttgcatcc	gactctcttt	tagacttatc	1900
1901	tccaatcaag	ccacaatttg	ctaaaggtac	tgacttcgtt	gttgtcagag	1950
1951	aattagtggg	aggtatttac	tttggtaaga	gaaaggaaga	cgatggtgat	2000
2001	ggtgtcgctt	gggatagtga	acaatacacc	gttccagaag	tgcaaagaat	2050
2051	cacaagaatg	gccgctttca	tggccctaca	acatgagcca	ccattgccta	2100
2101	tttggtcctt	ggataaagct	aatgttttgg	cctcttcaag	attatggaga	2150
2151	aaaactgtgg	aggaaaccat	caagaacgaa	ttccctacat	tgaaggttca	2200
2201	acatcaattg	attgattctg	ccgccatgat	cctagttaag	aacccaaccc	2250
2251	acctaaatgg	tattataatc	accagcaaca	tgtttggtga	tatcatctcc	2300
2301	gatgaagcct	ccgttatccc	aggttccttg	ggtttgttgc	catctgcgtc	2350
2351	cttggcctct	ttgccagaca	agaacaccgc	atttggtttg	tacgaaccat	2400
2401	gccacggttc	tgctccagat	ttgccaaaga	ataaggtcaa	ccctatcgcc	2450
2451	actatcttgt	ctgctgcaat	gatgttgaaa	ttgtcattga	acttgcctga	2500
2501	agaaggtaag	gccattgaag	atgcagttaa	aaaggttttg	gatgcaggta	2550
2551	tcagaactgg	tgatttaggt	ggttccaaca	gtaccacgga	agtcggtgat	2600
2601	gctgtcgccg	aagaagttaa	gaaaatcctt	gcttaaaaag	attctctttt	2650
2651	tttatgatat	ttgtacataa	actttataaa	tgaaattcat	aatagaaacg	2700
2701	acacgaaatt	acaaaatgga	atatgttcat	agggtagacg	aaactatata	2750
2751	cgcaatctac	atacatttat	caagaaggag	aaaaaggagg	atgtaaagga	2800
2801	atacaggtaa	gcaaattgat	actaatggct	caacgtgata	aggaaaaaga	2850
2851	attgcacttt	aacattaata	ttgacaagga	ggagggcacc	acacaaaaag	2900
2901	ttaggtgtaa	cagaaaatca	tgaaactatg	attcctaatt	tatatattgg	2950
2951	aggattttct	ctaaaaaaaa	aaaaatacaa	caaataaaaa	acactcaatg	3000
3001	acctgaccat	ttgatggagt	ttaagtcaat	accttcttga	accatttccc	3050
3051	ataatggtga	aagttccctc	aagaatttta	ctctgtcaga	aacggcctta	3100
3101	acgacgtagt	cgacctcctc	ttcagtacta	aatctaccaa	taccaaatct	3150
3151	gatggaagaa	tgggctaatg	catcatcctt	acccagcgca	tgtaaaacat	3200
3201	aagaaggttc	tagggaagca	gatgtacagg	ctgaacccga	ggataatgcg	3250
3251	atatccctta	gtgccatcaa	taaagattct	ccttccacgt	aggcgaaaga	3300
3301	aacgttaaca	caccctggat	aacgatgatc	tggagatccg	ttcaacgtgg	3350
3351	tatgttcagc	ggataataga	cctttgacta	atttatcgga	tagtcttttg	3400
3401	atgtgagctt	ggtcgttgtc	aaattctttc	ttcatcaatc	tcgcagcttc	3450
3451	accaaatccc	gctaccaatg	ggggggccaa	agtaccagat	ctgctgcatt	3500
3501	aatgaatcgg	ccaacgcgcg	gggagaggcg	gtttgcgtat	tgggcgctct	3550
3551	tccgcttcct	cgctcactga	ctcgctgcgc	tcggtcgttc	ggctgcggcg	3600
3601	agcggtatca	gcatcgatgc	atactttgtg	aacagaaagt	gatagcgttg	3650
3651	atgattcttc	attggtcaga	aaattatgaa	cggtttcttc	tattttgtct	3700
3701	ctatatacta	cgtataggaa	atgtttacat	tttcgtattg	ttttcgattc	3750
3751	actctatgaa	tagttcttac	tacaattttt	ttgtctaaag	agtaatacta	3800
3801	gagataaaca	taaaaaatgt	agaggtcgag	tttagatgca	agttcaagga	3850
3851	gcgaaaggtg	gatgggtagg	ttatataggg	atatagcaca	gagatatata	3900
3901	gcaaagagat	acttttgagc	aatgtttgtg	gaagcggtat	tcgcaatatt	3950
3951	ttagtagctc	gttacagtcc	ggtgcgtttt	tggttttttg	aaagtgcgtc	4000
4001	ttcagagcgc	ttttggtttt	caaaagcgct	ctgaagttcc	tatactttct	4050
4051	agctagagaa	taggaacttc	ggaataggaa	cttcaaagcg	tttccgaaaa	4100
4101	cgagcgcttc	cgaaaatgca	acgcgagctg	ccatcgatgc	tcactcaaag	4150
4151	gtcggtaata	cggttatcca	cagaatcagg	ggataacgca	ggaaagaaca	4200
4201	tgtgagcaaa	aggccagcaa	aaggccagga	accgtaaaaa	ggccgcgttg	4250
4251	ctggcgtttt	tccataggct	ccgcccccct	gacgagcatc	acaaaaatcg	4300
4301	acgctcaagt	cagaggtggc	gaaacccgac	aggactataa	agataccagg	4350
4351	cgtttccccc	tggaagctcc	ctcgtgcgct	ctcctgttcc	gaccctgccg	4400
4401	cttaccggat	acctgtccgc	ctttctccct	tcgggaagcg	tggcgctttc	4450
4451	tcatagctca	cgctgtaggt	atctcagttc	ggtgtaggtc	gttcgctcca	4500
4501	agctgggctg	tgtgcacgaa	ccccccgttc	agcccgaccg	ctgcgcctta	4550
4551	tccggtaact	atcgtcttga	gtccaacccg	gtaagacacg	acttatcgcc	4600
4601	actggcagca	gccactggta	acaggattag	cagagcgagg	tatgtaggcg	4650
4651	gtgctacaga	gttcttgaag	tggtggccta	actacggcta	cactagaagg	4700
4701	acagtatttg	gtatctgcgc	tctgctgaag	ccagttacct	tcggaaaaag	4750
4751	agttggtagc	tcttgatccg	gcaaacaaac	caccgctggt	agcggtggtt	4800
4801	tttttgtttg	caagcagcag	attacgcgca	gaaaaaaagg	atctcaagaa	4850
4851	gatcctttga	tcttttctac	ggggtctgac	gctcagtgga	acgaaaactc	4900
4901	acgttaaggg	attttggtca	tgagattatc	aaaaaggatc	ttcacctaga	4950
4951	tccttttaaa	ttaaaaatga	agttttaaat	caatctaaag	tatatatgag	5000
5001	taaacttggt	ctgacagtta	ccaatgctta	atcagtgagg	cacctatctc	5050
5051	agcgatctgt	ctatttcgtt	catccatagt	tgcctgactc	cccgtcgtgt	5100
5101	agataactac	gatacgggag	ggcttaccat	ctggccccag	tgctgcaatg	5150
5151	ataccgcgag	acccacgctc	accggctcca	gatttatcag	caataaacca	5200
5201	gccagccgga	agggccgagc	gcagaagtgg	tcctgcaact	ttatccgcct	5250
5251	ccatccagtc	tattaattgt	tgccgggaag	ctagagtaag	tagttcgcca	5300
5301	gttaatagtt	tgcgcaacgt	tgttgccatt	gctacaggca	tcgtggtgtc	5350
5351	acgctcgtcg	tttggtatgg	cttcattcag	ctccggttcc	caacgatcaa	5400
5401	ggcgagttac	atgatccccc	atgttgtgca	aaaaagcggt	tagctccttc	5450
5451	ggtcctccga	tcgttgtcag	aagtaagttg	gccgcagtgt	tatcactcat	5500
5501	ggttatggca	gcactgcata	attctcttac	tgtcatgcca	tccgtaagat	5550
5551	gcttttctgt	gactggtgag	tactcaacca	agtcattctg	agaatagtgt	5600
5601	atgcggcgac	cgagttgctc	ttgcccggcg	tcaatacggg	ataataccgc	5650
5651	gccacatagc	agaactttaa	aagtgctcat	cattggaaaa	cgttcttcgg	5700
5701	ggcgaaaact	ctcaaggatc	ttaccgctgt	tgagatccag	ttcgatgtaa	5750
5751	cccactcgtg	cacccaactg	atcttcagca	tcttttactt	tcaccagcgt	5800
5801	ttctgggtga	gcaaaaacag	gaaggcaaaa	tgccgcaaaa	aagggaataa	5850
5851	gggcgacacg	gaaatgttga	atactcatac	tcttcctttt	tcaatattat	5900
5901	tgaagcattt	atcagggtta	ttgtctcatg	agcggataca	tatttgaatg	5950
5951	tatttagaaa	aataaacaaa	taggggttcc	gcgcacattt	ccccgaaaag	6000
6001	tgccacctga	cgtctaagaa	accattatta	tcatgacatt	aacctataaa	6050
6051	aataggcgta	tcacgaggcc	ctttcgtc	6078