pFL36

保存条件 ：低温运输，-20℃保存

保质期 ：1年

英文名 ：pFL36

库存 ：大量

供应商 ：信裕生物

名称	编号	规格	价格	手册
pFL36

质粒编号:
质粒名称: pFL36
其他名称:
质粒大小（bp）: 6369
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号: X70481
其他属性: pFL36 was constructed from pUC19 plasmid where two alu I sites were modified. The site 629 was replaced by a BglII linker and the site 747 by a ClaI site. The yeast selectable marker has been cloned in the BglII site and the yeast replication origin in the ClaI site. The order of the major features in this plasmid is:EcoRI/MCS/HindIII – LEU2 – ARS-CEN6 – pMB1 ori – ampR. YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 8.3. Restriction digests of the clone give the following sizes (kb): BamHI–6.6; BglII–3.7, 2.9; ClaI–3.9, 1.9, 0.8. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 397129 Hosts: E.coli HB101, S.cerevisiae, E.coli, Saccharomyces. Related vectors: pUC19, yeast 2-micron plasmid.
质粒图谱:

质粒序列:

1	tcgcgcgttt	cggtgatgac	ggtgaaaacc	tctgacacat	gcagctcccg	50
51	gagacggtca	cagcttgtct	gtaagcggat	gccgggagca	gacaagcccg	100
101	tcagggcgcg	tcagcgggtg	ttggcgggtg	tcggggctgg	cttaactatg	150
151	cggcatcaga	gcagattgta	ctgagagtgc	accatatgcg	gtgtgaaata	200
201	ccgcacagat	gcgtaaggag	aaaataccgc	atcaggcgcc	attcgccatt	250
251	caggctgcgc	aactgttggg	aagggcgatc	ggtgcgggcc	tcttcgctat	300
301	tacgccagct	ggcgaaaggg	ggatgtgctg	caaggcgatt	aagttgggta	350
351	acgccagggt	tttcccagtc	acgacgttgt	aaaacgacgg	ccagtgaatt	400
401	cgagctcggt	acccggggat	cctctagagt	cgacctgcag	gcatgcaagc	450
451	ttggcgtaat	catggtcata	gctgtttcct	gtgtgaaatt	gttatccgct	500
501	cacaattcca	cacaacatac	gagccggaag	cataaagtgt	aaagcctggg	550
551	gtgcctaatg	agtgagctaa	ctcacattaa	ttgcgttgcg	ctcactgccc	600
601	gctttccagt	cgggaaacct	gtcgtgccag	cagatctatt	acattatggg	650
651	tggtatgttg	gaataaaaat	caactatcat	ctactaacta	gtatttacgt	700
701	tactagtata	ttatcatata	cggtgttaga	agatgacgca	aatgatgaga	750
751	aatagtcatc	taaattagtg	gaagctgaaa	cgcaaggatt	gataatgtaa	800
801	taggatcaat	gaatattaac	atataaaatg	atgataataa	tatttataga	850
851	attgtgtaga	attgcagatt	cccttttatg	gattcctaaa	tcctcgagga	900
901	gaacttctag	tatatctaca	tacctaatat	tattgcctta	ttaaaaatgg	950
951	aatcccaaca	attacatcaa	aatccacatt	ctcttcaaaa	tcaattgtcc	1000
1001	tgtacttcct	tgttcatgtg	tgttcaaaaa	cgttatattt	ataggataat	1050
1051	tatactctat	ttctcaacaa	gtaattggtt	gtttggccga	gcggtctaag	1100
1101	gcgcctgatt	caagaaatat	cttgaccgca	gttaactgtg	ggaatactca	1150
1151	ggtatcgtaa	gatgcaagag	ttcgaatctc	ttagcaacca	ttattttttt	1200
1201	cctcaacata	acgagaacac	acaggggcgc	tatcgcacag	aatcaaattc	1250
1251	gatgactgga	aattttttgt	taatttcaga	ggtcgcctga	cgcatatacc	1300
1301	tttttcaact	gaaaaattgg	gagaaaaagg	aaaggtgaga	gccgcggaac	1350
1351	cggcttttca	tatagaatag	agaagcgttc	atgactaaat	gcttgcatca	1400
1401	caatacttga	agttgacaat	attatttaag	gacctattgt	tttttccaat	1450
1451	aggtggttag	caatcgtctt	actttctaac	ttttcttacc	ttttacattt	1500
1501	cagcaatata	tatatatata	tttcaaggat	ataccattct	aatgtctgcc	1550
1551	cctaagaaga	tcgtcgtttt	gccaggtgac	cacgttggtc	aagaaatcac	1600
1601	agccgaagcc	attaaggttc	ttaaagctat	ttctgatgtt	cgttccaatg	1650
1651	tcaagttcga	tttcgaaaat	catttaattg	gtggtgctgc	tatcgatgct	1700
1701	acaggtgtcc	cacttccaga	tgaggcgctg	gaagcctcca	agaaggttga	1750
1751	tgccgttttg	ttaggtgctg	tgggtggtcc	taaatggggt	accggtagtg	1800
1801	ttagacctga	acaaggttta	ctaaaaatcc	gtaaagaact	tcaattgtac	1850
1851	gccaacttaa	gaccatgtaa	ctttgcatcc	gactctcttt	tagacttatc	1900
1901	tccaatcaag	ccacaatttg	ctaaaggtac	tgacttcgtt	gttgtcagag	1950
1951	aattagtggg	aggtatttac	tttggtaaga	gaaaggaaga	cgatggtgat	2000
2001	ggtgtcgctt	gggatagtga	acaatacacc	gttccagaag	tgcaaagaat	2050
2051	cacaagaatg	gccgctttca	tggccctaca	acatgagcca	ccattgccta	2100
2101	tttggtcctt	ggataaagct	aatgttttgg	cctcttcaag	attatggaga	2150
2151	aaaactgtgg	aggaaaccat	caagaacgaa	ttccctacat	tgaaggttca	2200
2201	acatcaattg	attgattctg	ccgccatgat	cctagttaag	aacccaaccc	2250
2251	acctaaatgg	tattataatc	accagcaaca	tgtttggtga	tatcatctcc	2300
2301	gatgaagcct	ccgttatccc	aggttccttg	ggtttgttgc	catctgcgtc	2350
2351	cttggcctct	ttgccagaca	agaacaccgc	atttggtttg	tacgaaccat	2400
2401	gccacggttc	tgctccagat	ttgccaaaga	ataaggtcaa	ccctatcgcc	2450
2451	actatcttgt	ctgctgcaat	gatgttgaaa	ttgtcattga	acttgcctga	2500
2501	agaaggtaag	gccattgaag	atgcagttaa	aaaggttttg	gatgcaggta	2550
2551	tcagaactgg	tgatttaggt	ggttccaaca	gtaccacgga	agtcggtgat	2600
2601	gctgtcgccg	aagaagttaa	gaaaatcctt	gcttaaaaag	attctctttt	2650
2651	tttatgatat	ttgtacataa	actttataaa	tgaaattcat	aatagaaacg	2700
2701	acacgaaatt	acaaaatgga	atatgttcat	agggtagacg	aaactatata	2750
2751	cgcaatctac	atacatttat	caagaaggag	aaaaaggagg	atgtaaagga	2800
2801	atacaggtaa	gcaaattgat	actaatggct	caacgtgata	aggaaaaaga	2850
2851	attgcacttt	aacattaata	ttgacaagga	ggagggcacc	acacaaaaag	2900
2901	ttaggtgtaa	cagaaaatca	tgaaactatg	attcctaatt	tatatattgg	2950
2951	aggattttct	ctaaaaaaaa	aaaaatacaa	caaataaaaa	acactcaatg	3000
3001	acctgaccat	ttgatggagt	ttaagtcaat	accttcttga	accatttccc	3050
3051	ataatggtga	aagttccctc	aagaatttta	ctctgtcaga	aacggcctta	3100
3101	acgacgtagt	cgacctcctc	ttcagtacta	aatctaccaa	taccaaatct	3150
3151	gatggaagaa	tgggctaatg	catcatcctt	acccagcgca	tgtaaaacat	3200
3201	aagaaggttc	tagggaagca	gatgtacagg	ctgaacccga	ggataatgcg	3250
3251	atatccctta	gtgccatcaa	taaagattct	ccttccacgt	aggcgaaaga	3300
3301	aacgttaaca	caccctggat	aacgatgatc	tggagatccg	ttcaacgtgg	3350
3351	tatgttcagc	ggataataga	cctttgacta	atttatcgga	tagtcttttg	3400
3401	atgtgagctt	ggtcgttgtc	aaattctttc	ttcatcaatc	tcgcagcttc	3450
3451	accaaatccc	gctaccaatg	ggggggccaa	agtaccagat	ctgctgcatt	3500
3501	aatgaatcgg	ccaacgcgcg	gggagaggcg	gtttgcgtat	tgggcgctct	3550
3551	tccgcttcct	cgctcactga	ctcgctgcgc	tcggtcgttc	ggctgcggcg	3600
3601	agcggtatca	gcatcgatga	atggaaatta	cgtactttca	acgcaagagc	3650
3651	aagacaaaag	aaagcctaaa	actgtctttt	cgtgtcggtc	gtccaatatc	3700
3701	atcgtaaacg	tgtgtagagc	cttaaattct	aatttctaac	tttaaatttt	3750
3751	ttttgctatt	ataatactaa	tttctaactt	tcaacctatt	ttacatcttc	3800
3801	ggaaaacaaa	aactattttt	tctttaattt	ctttttttac	tttctatttt	3850
3851	taatttatat	atttatatta	aaaaatttaa	attatattat	ttttatagca	3900
3901	cgtgatgaaa	agaaatatag	tcttcaaggt	tatattatac	aggtttatat	3950
3951	attattttac	ttatttttgt	tgttcttttt	cttggagtaa	tttagcatta	4000
4001	acaacttcga	cccatcgcga	tgacgaaatt	gcacgtttaa	aggagaagtc	4050
4051	ggaatcgttg	caagaagaaa	taaattagtt	tgacgtttta	ttccttttct	4100
4101	tctgcacaga	agtgtgtata	tatatattta	tatatagtcg	atgtgcaaaa	4150
4151	gcctacatta	tttttctttt	gatttttatt	tttttgttca	agtaggttca	4200
4201	tgccgacagt	aaatccttat	gccctaccta	aatataaaca	cccaaatata	4250
4251	tatatatata	tatatatata	taaataataa	aaacggaaag	gaaatagaat	4300
4301	aataataata	atactacacg	tttacgtatg	taactcattc	tattattcag	4350
4351	actttgatat	taatagaatt	attactacta	ggtttcctca	cttcaaaatc	4400
4401	tataaatata	ccgtcatgct	ctaatcgatg	ctcactcaaa	ggtcggtaat	4450
4451	acggttatcc	acagaatcag	gggataacgc	aggaaagaac	atgtgagcaa	4500
4501	aaggccagca	aaaggccagg	aaccgtaaaa	aggccgcgtt	gctggcgttt	4550
4551	ttccataggc	tccgcccccc	tgacgagcat	cacaaaaatc	gacgctcaag	4600
4601	tcagaggtgg	cgaaacccga	caggactata	aagataccag	gcgtttcccc	4650
4651	ctggaagctc	cctcgtgcgc	tctcctgttc	cgaccctgcc	gcttaccgga	4700
4701	tacctgtccg	cctttctccc	ttcgggaagc	gtggcgcttt	ctcatagctc	4750
4751	acgctgtagg	tatctcagtt	cggtgtaggt	cgttcgctcc	aagctgggct	4800
4801	gtgtgcacga	accccccgtt	cagcccgacc	gctgcgcctt	atccggtaac	4850
4851	tatcgtcttg	agtccaaccc	ggtaagacac	gacttatcgc	cactggcagc	4900
4901	agccactggt	aacaggatta	gcagagcgag	gtatgtaggc	ggtgctacag	4950
4951	agttcttgaa	gtggtggcct	aactacggct	acactagaag	gacagtattt	5000
5001	ggtatctgcg	ctctgctgaa	gccagttacc	ttcggaaaaa	gagttggtag	5050
5051	ctcttgatcc	ggcaaacaaa	ccaccgctgg	tagcggtggt	ttttttgttt	5100
5101	gcaagcagca	gattacgcgc	agaaaaaaag	gatctcaaga	agatcctttg	5150
5151	atcttttcta	cggggtctga	cgctcagtgg	aacgaaaact	cacgttaagg	5200
5201	gattttggtc	atgagattat	caaaaaggat	cttcacctag	atccttttaa	5250
5251	attaaaaatg	aagttttaaa	tcaatctaaa	gtatatatga	gtaaacttgg	5300
5301	tctgacagtt	accaatgctt	aatcagtgag	gcacctatct	cagcgatctg	5350
5351	tctatttcgt	tcatccatag	ttgcctgact	ccccgtcgtg	tagataacta	5400
5401	cgatacggga	gggcttacca	tctggcccca	gtgctgcaat	gataccgcga	5450
5451	gacccacgct	caccggctcc	agatttatca	gcaataaacc	agccagccgg	5500
5501	aagggccgag	cgcagaagtg	gtcctgcaac	tttatccgcc	tccatccagt	5550
5551	ctattaattg	ttgccgggaa	gctagagtaa	gtagttcgcc	agttaatagt	5600
5601	ttgcgcaacg	ttgttgccat	tgctacaggc	atcgtggtgt	cacgctcgtc	5650
5651	gtttggtatg	gcttcattca	gctccggttc	ccaacgatca	aggcgagtta	5700
5701	catgatcccc	catgttgtgc	aaaaaagcgg	ttagctcctt	cggtcctccg	5750
5751	atcgttgtca	gaagtaagtt	ggccgcagtg	ttatcactca	tggttatggc	5800
5801	agcactgcat	aattctctta	ctgtcatgcc	atccgtaaga	tgcttttctg	5850
5851	tgactggtga	gtactcaacc	aagtcattct	gagaatagtg	tatgcggcga	5900
5901	ccgagttgct	cttgcccggc	gtcaatacgg	gataataccg	cgccacatag	5950
5951	cagaacttta	aaagtgctca	tcattggaaa	acgttcttcg	gggcgaaaac	6000
6001	tctcaaggat	cttaccgctg	ttgagatcca	gttcgatgta	acccactcgt	6050
6051	gcacccaact	gatcttcagc	atcttttact	ttcaccagcg	tttctgggtg	6100
6101	agcaaaaaca	ggaaggcaaa	atgccgcaaa	aaagggaata	agggcgacac	6150
6151	ggaaatgttg	aatactcata	ctcttccttt	ttcaatatta	ttgaagcatt	6200
6201	tatcagggtt	attgtctcat	gagcggatac	atatttgaat	gtatttagaa	6250
6251	aaataaacaa	ataggggttc	cgcgcacatt	tccccgaaaa	gtgccacctg	6300
6301	acgtctaaga	aaccattatt	atcatgacat	taacctataa	aaataggcgt	6350
6351	atcacgaggc	cctttcgtc	6369