产品详情
文献和实验
相关推荐
保存条件 :低温运输,-20℃保存
保质期 :1年
英文名 :pET-28a(+)
库存 :大量
供应商 :信裕生物
质粒编号: 60908-2793y
质粒名称: pET-28a(+)
其他名称:
质粒大小(bp): 5369
抗性: Kanamycin
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-His, N-T7, C-His
5’测序引物: T7: TAATACGACTCACTATAGGG
3′ 测序引物:T7ter: GCTAGTTATTGCTCAGCGG
GenBank号:
其他属性: Nterm thrombin cleavage site; a,b,c vary by MCS;
质粒图谱:
Feature Name | Start | End | |
---|---|---|---|
T7_terminator | 129 | 1 | |
T7_Terminal_primer | 69 | 87 | |
T7_leader | 239 | 207 | |
Xpress_fwd_primer | 240 | 222 | |
T7_transl_en_RBS | 323 | 307 | |
lacO | 368 | 341 | |
T7_promoter | 386 | 368 | |
tet (300 – 563) | 418 | 681 | |
pBRrevBam_primer | 489 | 470 | |
lacI | 764 | 1855 | |
tet (576 – 851) | 1914 | 2189 | |
ROP | 2664 | 2855 | |
pGEX_3_primer | 2871 | 2849 | |
pBR322_origin | 3889 | 3270 | |
KanR2 | 3995 | 4810 |
ORF | Start | End | |
---|---|---|---|
ORF frame 1 | 523 | 1023 | |
ORF frame 2 | 896 | 1855 | |
ORF frame 2 | 3995 | 4810 |
Enzyme Name | Cut |
---|---|
XhoI | 158 |
NotI | 166 |
EagI | 166 |
HindIII | 173 |
SalI | 179 |
SacI | 190 |
EcoRI | 192 |
BamHI | 198 |
NheI | 231 |
NdeI | 238 |
NcoI | 296 |
XbaI | 335 |
BglII | 401 |
BclI | 1137 |
ApaI | 1334 |
EcoRV | 1573 |
HpaI | 1629 |
FspI | 2205 |
NruI | 4083 |
ClaI | 4117 |
XmaI | 4298 |
SmaI | 4300 |
质粒序列:
1 | ATCCGGATAT | AGTTCCTCCT | TTCAGCAAAA | AACCCCTCAA | GACCCGTTTA | 50 |
51 | GAGGCCCCAA | GGGGTTATGC | TAGTTATTGC | TCAGCGGTGG | CAGCAGCCAA | 100 |
101 | CTCAGCTTCC | TTTCGGGCTT | TGTTAGCAGC | CGGATCTCAG | TGGTGGTGGT | 150 |
151 | GGTGGTGCTC | GAGTGCGGCC | GCAAGCTTGT | CGACGGAGCT | CGAATTCGGA | 200 |
201 | TCCGCGACCC | ATTTGCTGTC | CACCAGTCAT | GCTAGCCATA | TGGCTGCCGC | 250 |
251 | GCGGCACCAG | GCCGCTGCTG | TGATGATGAT | GATGATGGCT | GCTGCCCATG | 300 |
301 | GTATATCTCC | TTCTTAAAGT | TAAACAAAAT | TATTTCTAGA | GGGGAATTGT | 350 |
351 | TATCCGCTCA | CAATTCCCCT | ATAGTGAGTC | GTATTAATTT | CGCGGGATCG | 400 |
401 | AGATCTCGAT | CCTCTACGCC | GGACGCATCG | TGGCCGGCAT | CACCGGCGCC | 450 |
451 | ACAGGTGCGG | TTGCTGGCGC | CTATATCGCC | GACATCACCG | ATGGGGAAGA | 500 |
501 | TCGGGCTCGC | CACTTCGGGC | TCATGAGCGC | TTGTTTCGGC | GTGGGTATGG | 550 |
551 | TGGCAGGCCC | CGTGGCCGGG | GGACTGTTGG | GCGCCATCTC | CTTGCATGCA | 600 |
601 | CCATTCCTTG | CGGCGGCGGT | GCTCAACGGC | CTCAACCTAC | TACTGGGCTG | 650 |
651 | CTTCCTAATG | CAGGAGTCGC | ATAAGGGAGA | GCGTCGAGAT | CCCGGACACC | 700 |
701 | ATCGAATGGC | GCAAAACCTT | TCGCGGTATG | GCATGATAGC | GCCCGGAAGA | 750 |
751 | GAGTCAATTC | AGGGTGGTGA | ATGTGAAACC | AGTAACGTTA | TACGATGTCG | 800 |
801 | CAGAGTATGC | CGGTGTCTCT | TATCAGACCG | TTTCCCGCGT | GGTGAACCAG | 850 |
851 | GCCAGCCACG | TTTCTGCGAA | AACGCGGGAA | AAAGTGGAAG | CGGCGATGGC | 900 |
901 | GGAGCTGAAT | TACATTCCCA | ACCGCGTGGC | ACAACAACTG | GCGGGCAAAC | 950 |
951 | AGTCGTTGCT | GATTGGCGTT | GCCACCTCCA | GTCTGGCCCT | GCACGCGCCG | 1000 |
1001 | TCGCAAATTG | TCGCGGCGAT | TAAATCTCGC | GCCGATCAAC | TGGGTGCCAG | 1050 |
1051 | CGTGGTGGTG | TCGATGGTAG | AACGAAGCGG | CGTCGAAGCC | TGTAAAGCGG | 1100 |
1101 | CGGTGCACAA | TCTTCTCGCG | CAACGCGTCA | GTGGGCTGAT | CATTAACTAT | 1150 |
1151 | CCGCTGGATG | ACCAGGATGC | CATTGCTGTG | GAAGCTGCCT | GCACTAATGT | 1200 |
1201 | TCCGGCGTTA | TTTCTTGATG | TCTCTGACCA | GACACCCATC | AACAGTATTA | 1250 |
1251 | TTTTCTCCCA | TGAAGACGGT | ACGCGACTGG | GCGTGGAGCA | TCTGGTCGCA | 1300 |
1301 | TTGGGTCACC | AGCAAATCGC | GCTGTTAGCG | GGCCCATTAA | GTTCTGTCTC | 1350 |
1351 | GGCGCGTCTG | CGTCTGGCTG | GCTGGCATAA | ATATCTCACT | CGCAATCAAA | 1400 |
1401 | TTCAGCCGAT | AGCGGAACGG | GAAGGCGACT | GGAGTGCCAT | GTCCGGTTTT | 1450 |
1451 | CAACAAACCA | TGCAAATGCT | GAATGAGGGC | ATCGTTCCCA | CTGCGATGCT | 1500 |
1501 | GGTTGCCAAC | GATCAGATGG | CGCTGGGCGC | AATGCGCGCC | ATTACCGAGT | 1550 |
1551 | CCGGGCTGCG | CGTTGGTGCG | GATATCTCGG | TAGTGGGATA | CGACGATACC | 1600 |
1601 | GAAGACAGCT | CATGTTATAT | CCCGCCGTTA | ACCACCATCA | AACAGGATTT | 1650 |
1651 | TCGCCTGCTG | GGGCAAACCA | GCGTGGACCG | CTTGCTGCAA | CTCTCTCAGG | 1700 |
1701 | GCCAGGCGGT | GAAGGGCAAT | CAGCTGTTGC | CCGTCTCACT | GGTGAAAAGA | 1750 |
1751 | AAAACCACCC | TGGCGCCCAA | TACGCAAACC | GCCTCTCCCC | GCGCGTTGGC | 1800 |
1801 | CGATTCATTA | ATGCAGCTGG | CACGACAGGT | TTCCCGACTG | GAAAGCGGGC | 1850 |
1851 | AGTGAGCGCA | ACGCAATTAA | TGTAAGTTAG | CTCACTCATT | AGGCACCGGG | 1900 |
1901 | ATCTCGACCG | ATGCCCTTGA | GAGCCTTCAA | CCCAGTCAGC | TCCTTCCGGT | 1950 |
1951 | GGGCGCGGGG | CATGACTATC | GTCGCCGCAC | TTATGACTGT | CTTCTTTATC | 2000 |
2001 | ATGCAACTCG | TAGGACAGGT | GCCGGCAGCG | CTCTGGGTCA | TTTTCGGCGA | 2050 |
2051 | GGACCGCTTT | CGCTGGAGCG | CGACGATGAT | CGGCCTGTCG | CTTGCGGTAT | 2100 |
2101 | TCGGAATCTT | GCACGCCCTC | GCTCAAGCCT | TCGTCACTGG | TCCCGCCACC | 2150 |
2151 | AAACGTTTCG | GCGAGAAGCA | GGCCATTATC | GCCGGCATGG | CGGCCCCACG | 2200 |
2201 | GGTGCGCATG | ATCGTGCTCC | TGTCGTTGAG | GACCCGGCTA | GGCTGGCGGG | 2250 |
2251 | GTTGCCTTAC | TGGTTAGCAG | AATGAATCAC | CGATACGCGA | GCGAACGTGA | 2300 |
2301 | AGCGACTGCT | GCTGCAAAAC | GTCTGCGACC | TGAGCAACAA | CATGAATGGT | 2350 |
2351 | CTTCGGTTTC | CGTGTTTCGT | AAAGTCTGGA | AACGCGGAAG | TCAGCGCCCT | 2400 |
2401 | GCACCATTAT | GTTCCGGATC | TGCATCGCAG | GATGCTGCTG | GCTACCCTGT | 2450 |
2451 | GGAACACCTA | CATCTGTATT | AACGAAGCGC | TGGCATTGAC | CCTGAGTGAT | 2500 |
2501 | TTTTCTCTGG | TCCCGCCGCA | TCCATACCGC | CAGTTGTTTA | CCCTCACAAC | 2550 |
2551 | GTTCCAGTAA | CCGGGCATGT | TCATCATCAG | TAACCCGTAT | CGTGAGCATC | 2600 |
2601 | CTCTCTCGTT | TCATCGGTAT | CATTACCCCC | ATGAACAGAA | ATCCCCCTTA | 2650 |
2651 | CACGGAGGCA | TCAGTGACCA | AACAGGAAAA | AACCGCCCTT | AACATGGCCC | 2700 |
2701 | GCTTTATCAG | AAGCCAGACA | TTAACGCTTC | TGGAGAAACT | CAACGAGCTG | 2750 |
2751 | GACGCGGATG | AACAGGCAGA | CATCTGTGAA | TCGCTTCACG | ACCACGCTGA | 2800 |
2801 | TGAGCTTTAC | CGCAGCTGCC | TCGCGCGTTT | CGGTGATGAC | GGTGAAAACC | 2850 |
2851 | TCTGACACAT | GCAGCTCCCG | GAGACGGTCA | CAGCTTGTCT | GTAAGCGGAT | 2900 |
2901 | GCCGGGAGCA | GACAAGCCCG | TCAGGGCGCG | TCAGCGGGTG | TTGGCGGGTG | 2950 |
2951 | TCGGGGCGCA | GCCATGACCC | AGTCACGTAG | CGATAGCGGA | GTGTATACTG | 3000 |
3001 | GCTTAACTAT | GCGGCATCAG | AGCAGATTGT | ACTGAGAGTG | CACCATATAT | 3050 |
3051 | GCGGTGTGAA | ATACCGCACA | GATGCGTAAG | GAGAAAATAC | CGCATCAGGC | 3100 |
3101 | GCTCTTCCGC | TTCCTCGCTC | ACTGACTCGC | TGCGCTCGGT | CGTTCGGCTG | 3150 |
3151 | CGGCGAGCGG | TATCAGCTCA | CTCAAAGGCG | GTAATACGGT | TATCCACAGA | 3200 |
3201 | ATCAGGGGAT | AACGCAGGAA | AGAACATGTG | AGCAAAAGGC | CAGCAAAAGG | 3250 |
3251 | CCAGGAACCG | TAAAAAGGCC | GCGTTGCTGG | CGTTTTTCCA | TAGGCTCCGC | 3300 |
3301 | CCCCCTGACG | AGCATCACAA | AAATCGACGC | TCAAGTCAGA | GGTGGCGAAA | 3350 |
3351 | CCCGACAGGA | CTATAAAGAT | ACCAGGCGTT | TCCCCCTGGA | AGCTCCCTCG | 3400 |
3401 | TGCGCTCTCC | TGTTCCGACC | CTGCCGCTTA | CCGGATACCT | GTCCGCCTTT | 3450 |
3451 | CTCCCTTCGG | GAAGCGTGGC | GCTTTCTCAT | AGCTCACGCT | GTAGGTATCT | 3500 |
3501 | CAGTTCGGTG | TAGGTCGTTC | GCTCCAAGCT | GGGCTGTGTG | CACGAACCCC | 3550 |
3551 | CCGTTCAGCC | CGACCGCTGC | GCCTTATCCG | GTAACTATCG | TCTTGAGTCC | 3600 |
3601 | AACCCGGTAA | GACACGACTT | ATCGCCACTG | GCAGCAGCCA | CTGGTAACAG | 3650 |
3651 | GATTAGCAGA | GCGAGGTATG | TAGGCGGTGC | TACAGAGTTC | TTGAAGTGGT | 3700 |
3701 | GGCCTAACTA | CGGCTACACT | AGAAGGACAG | TATTTGGTAT | CTGCGCTCTG | 3750 |
3751 | CTGAAGCCAG | TTACCTTCGG | AAAAAGAGTT | GGTAGCTCTT | GATCCGGCAA | 3800 |
3801 | ACAAACCACC | GCTGGTAGCG | GTGGTTTTTT | TGTTTGCAAG | CAGCAGATTA | 3850 |
3851 | CGCGCAGAAA | AAAAGGATCT | CAAGAAGATC | CTTTGATCTT | TTCTACGGGG | 3900 |
3901 | TCTGACGCTC | AGTGGAACGA | AAACTCACGT | TAAGGGATTT | TGGTCATGAA | 3950 |
3951 | CAATAAAACT | GTCTGCTTAC | ATAAACAGTA | ATACAAGGGG | TGTTATGAGC | 4000 |
4001 | CATATTCAAC | GGGAAACGTC | TTGCTCTAGG | CCGCGATTAA | ATTCCAACAT | 4050 |
4051 | GGATGCTGAT | TTATATGGGT | ATAAATGGGC | TCGCGATAAT | GTCGGGCAAT | 4100 |
4101 | CAGGTGCGAC | AATCTATCGA | TTGTATGGGA | AGCCCGATGC | GCCAGAGTTG | 4150 |
4151 | TTTCTGAAAC | ATGGCAAAGG | TAGCGTTGCC | AATGATGTTA | CAGATGAGAT | 4200 |
4201 | GGTCAGACTA | AACTGGCTGA | CGGAATTTAT | GCCTCTTCCG | ACCATCAAGC | 4250 |
4251 | ATTTTATCCG | TACTCCTGAT | GATGCATGGT | TACTCACCAC | TGCGATCCCC | 4300 |
4301 | GGGAAAACAG | CATTCCAGGT | ATTAGAAGAA | TATCCTGATT | CAGGTGAAAA | 4350 |
4351 | TATTGTTGAT | GCGCTGGCAG | TGTTCCTGCG | CCGGTTGCAT | TCGATTCCTG | 4400 |
4401 | TTTGTAATTG | TCCTTTTAAC | AGCGATCGCG | TATTTCGTCT | CGCTCAGGCG | 4450 |
4451 | CAATCACGAA | TGAATAACGG | TTTGGTTGAT | GCGAGTGATT | TTGATGACGA | 4500 |
4501 | GCGTAATGGC | TGGCCTGTTG | AACAAGTCTG | GAAAGAAATG | CATAAACTTT | 4550 |
4551 | TGCCATTCTC | ACCGGATTCA | GTCGTCACTC | ATGGTGATTT | CTCACTTGAT | 4600 |
4601 | AACCTTATTT | TTGACGAGGG | GAAATTAATA | GGTTGTATTG | ATGTTGGACG | 4650 |
4651 | AGTCGGAATC | GCAGACCGAT | ACCAGGATCT | TGCCATCCTA | TGGAACTGCC | 4700 |
4701 | TCGGTGAGTT | TTCTCCTTCA | TTACAGAAAC | GGCTTTTTCA | AAAATATGGT | 4750 |
4751 | ATTGATAATC | CTGATATGAA | TAAATTGCAG | TTTCATTTGA | TGCTCGATGA | 4800 |
4801 | GTTTTTCTAA | GAATTAATTC | ATGAGCGGAT | ACATATTTGA | ATGTATTTAG | 4850 |
4851 | AAAAATAAAC | AAATAGGGGT | TCCGCGCACA | TTTCCCCGAA | AAGTGCCACC | 4900 |
4901 | TGAAATTGTA | AACGTTAATA | TTTTGTTAAA | ATTCGCGTTA | AATTTTTGTT | 4950 |
4951 | AAATCAGCTC | ATTTTTTAAC | CAATAGGCCG | AAATCGGCAA | AATCCCTTAT | 5000 |
5001 | AAATCAAAAG | AATAGACCGA | GATAGGGTTG | AGTGTTGTTC | CAGTTTGGAA | 5050 |
5051 | CAAGAGTCCA | CTATTAAAGA | ACGTGGACTC | CAACGTCAAA | GGGCGAAAAA | 5100 |
5101 | CCGTCTATCA | GGGCGATGGC | CCACTACGTG | AACCATCACC | CTAATCAAGT | 5150 |
5151 | TTTTTGGGGT | CGAGGTGCCG | TAAAGCACTA | AATCGGAACC | CTAAAGGGAG | 5200 |
5201 | CCCCCGATTT | AGAGCTTGAC | GGGGAAAGCC | GGCGAACGTG | GCGAGAAAGG | 5250 |
5251 | AAGGGAAGAA | AGCGAAAGGA | GCGGGCGCTA | GGGCGCTGGC | AAGTGTAGCG | 5300 |
5301 | GTCACGCTGC | GCGTAACCAC | CACACCCGCC | GCGCTTAATG | CGCCGCTACA | 5350 |
5351 | GGGCGCGTCC | CATTCGCCA | 5369 |
上海信裕生物科技有限公司
品牌商实名认证
钻石会员
入驻年限:12年