pEMBL8+

保存条件 ：低温运输，-20℃保存

保质期 ：1年

英文名 ：pEMBL8+

库存 ：大量

供应商 ：信裕生物

名称	编号	规格	价格	手册
pEMBL8+

质粒编号:
质粒名称: pEMBL8+
其他名称:
质粒大小（bp）: 3939
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号: X04995, M19090
其他属性: According to the cloning strategy, the phage f1 origin should be flanked by regenerated EcoRI sites, but these are not found experimentally. The given sequence must be incorrect around this position. The base n was introduced to destroy the EcoRI site (GAAnTTC). ssDNA-producing plasmid with polylinker in lacZ'. Medium is 1227 LB plus ampicillin. Hosts: E.coli 71/18, E.coli, E.coli JM series. Related vectors: pUC8, M13mp8, pEMBL8-.
质粒图谱:

质粒序列:

1	gcccaatacg	caaaccgcct	ctccccgcgc	gttggccgat	tcattaatgc	50
51	agctggcacg	acaggtttcc	cgactggaaa	gcgggcagtg	agcgcaacgc	100
101	aattaatgtg	agttagctca	ctcattaggc	accccaggct	ttacacttta	150
151	tgcttccggc	tcgtatgttg	tgtggaattg	tgagcggata	acaatttcac	200
201	acaggaaaca	gctatgacca	tgattacgaa	ttcccgggga	tccgtcgacc	250
251	tgcagccaag	cttggcactg	gccgtcgttt	tacaacgtcg	tgactgggaa	300
301	aaccctggcg	ttacccaact	taatcgcctt	gcagcacatc	cccctttcgc	350
351	cagctggcgt	aatagcgaag	aggcccgcac	cgatcgccct	tcccaacagt	400
401	tgcgcagcct	gaatggcgaa	tggaanttcc	agacgattga	gcgtcaaaat	450
451	gtaggtattt	ccatgagcgt	ttttcctgtt	gcaatggctg	gcggtaatat	500
501	tgttctggat	attaccagca	aggccgatag	tttgagttct	tctactcagg	550
551	caagtgatgt	tattactaat	caaagaagta	ttgcgacaac	ggttaatttg	600
601	cgtgatggac	agactctttt	actcggtggc	ctcactgatt	ataaaaacac	650
651	ttctcaggat	tctggcgtac	cgttcctgtc	taaaatccct	ttaatcggcc	700
701	tcctgtttag	ctcccgctct	gattctaacg	aggaaagcac	gttatacgtg	750
751	ctcgtcaaag	caaccatagt	acgcgccctg	tagcggcgca	ttaagcgcgg	800
801	cgggtgtggt	ggttacgcgc	agcgtgaccg	ctacacttgc	cagcgcccta	850
851	gcgcccgctc	ctttcgcttt	cttcccttcc	tttctcgcca	cgttcgccgg	900
901	ctttccccgt	caagctctaa	atcgggggct	ccctttaggg	ttccgattta	950
951	gtgctttacg	gcacctcgac	cccaaaaaac	ttgattaggg	tgatggttca	1000
1001	cgtagtgggc	catcgccctg	atagacggtt	tttcgccctt	tgacgttgga	1050
1051	gtccacgttc	tttaatagtg	gactcttgtt	ccaaactgga	acaacactca	1100
1101	accctatctc	ggtctattct	tttgatttat	aagggatttt	gccgatttcg	1150
1151	gcctattggt	taaaaaatga	gctgatttaa	caaaaattta	acgcgaattt	1200
1201	taacaaaata	ttaacgttta	caatttaaat	atttgcttat	acaatcttcc	1250
1251	tgtttttggg	gcttttctga	ttatcaaccg	gggtacatat	gattgacatg	1300
1301	ctagttttac	gattaccgtt	catcgattct	cttgtttgct	ccagactctc	1350
1351	aggcaatgac	ctgatagcct	ttgtagagac	ctctcaaaaa	tagctaccct	1400
1401	ctccggcatg	aatttatcag	ctagaacggt	tgaatatcat	attgatggtg	1450
1451	atttgactgt	ctccggcctt	tctcacccgt	ttgaatcttt	acctacacat	1500
1501	tactcaggca	ttgcatttaa	aatatatgag	ggttctaaaa	atttttatcc	1550
1551	ttgcgttgaa	ataaaggctt	ctcccgcaaa	agtattacag	ggtcataatg	1600
1601	tttttggtac	aaccgattta	gctttatgct	ctgaggcttt	attgcttaat	1650
1651	tttgctaatt	ctttgccttg	cctgtatgat	ttattggatg	ttggaanttc	1700
1701	ctgatgcggt	attttctcct	tacgcatctg	tgcggtattt	cacaccgcat	1750
1751	atggtgcact	ctcagtacaa	tctgctctga	tgccgcatag	ttaagccagc	1800
1801	cccgacaccc	gccaacaccc	gctgacgcgc	cctgacgggc	ttgtctgctc	1850
1851	ccggcatccg	cttacagaca	agctgtgacc	gtctccggga	gctgcatgtg	1900
1901	tcagaggttt	tcaccgtcat	caccgaaacg	cgcgagacga	aagggcctcg	1950
1951	tgatacgcct	atttttatag	gttaatgtca	tgataataat	ggtttcttag	2000
2001	acgtcaggtg	gcacttttcg	gggaaatgtg	cgcggaaccc	ctatttgttt	2050
2051	atttttctaa	atacattcaa	atatgtatcc	gctcatgaga	caataaccct	2100
2101	gataaatgct	tcaataatat	tgaaaaagga	agagtatgag	tattcaacat	2150
2151	ttccgtgtcg	cccttattcc	cttttttgcg	gcattttgcc	ttcctgtttt	2200
2201	tgctcaccca	gaaacgctgg	tgaaagtaaa	agatgctgaa	gatcagttgg	2250
2251	gtgcacgagt	gggttacatc	gaactggatc	tcaacagcgg	taagatcctt	2300
2301	gagagttttc	gccccgaaga	acgttttcca	atgatgagca	cttttaaagt	2350
2351	tctgctatgt	ggcgcggtat	tatcccgtat	tgacgccggg	caagagcaac	2400
2401	tcggtcgccg	catacactat	tctcagaatg	acttggttga	gtactcacca	2450
2451	gtcacagaaa	agcatcttac	ggatggcatg	acagtaagag	aattatgcag	2500
2501	tgctgccata	accatgagtg	ataacactgc	ggccaactta	cttctgacaa	2550
2551	cgatcggagg	accgaaggag	ctaaccgctt	ttttgcacaa	catgggggat	2600
2601	catgtaactc	gccttgatcg	ttgggaaccg	gagctgaatg	aagccatacc	2650
2651	aaacgacgag	cgtgacacca	cgatgcctgt	agcaatggca	acaacgttgc	2700
2701	gcaaactatt	aactggcgaa	ctacttactc	tagcttcccg	gcaacaatta	2750
2751	atagactgga	tggaggcgga	taaagttgca	ggaccacttc	tgcgctcggc	2800
2801	ccttccggct	ggctggttta	ttgctgataa	atctggagcc	ggtgagcgtg	2850
2851	ggtctcgcgg	tatcattgca	gcactggggc	cagatggtaa	gccctcccgt	2900
2901	atcgtagtta	tctacacgac	ggggagtcag	gcaactatgg	atgaacgaaa	2950
2951	tagacagatc	gctgagatag	gtgcctcact	gattaagcat	tggtaactgt	3000
3001	cagaccaagt	ttactcatat	atactttaga	ttgatttaaa	acttcatttt	3050
3051	taatttaaaa	ggatctaggt	gaagatcctt	tttgataatc	tcatgaccaa	3100
3101	aatcccttaa	cgtgagtttt	cgttccactg	agcgtcagac	cccgtagaaa	3150
3151	agatcaaagg	atcttcttga	gatccttttt	ttctgcgcgt	aatctgctgc	3200
3201	ttgcaaacaa	aaaaaccacc	gctaccagcg	gtggtttgtt	tgccggatca	3250
3251	agagctacca	actctttttc	cgaaggtaac	tggcttcagc	agagcgcaga	3300
3301	taccaaatac	tgtccttcta	gtgtagccgt	agttaggcca	ccacttcaag	3350
3351	aactctgtag	caccgcctac	atacctcgct	ctgctaatcc	tgttaccagt	3400
3401	ggctgctgcc	agtggcgata	agtcgtgtct	taccgggttg	gactcaagac	3450
3451	gatagttacc	ggataaggcg	cagcggtcgg	gctgaacggg	gggttcgtgc	3500
3501	acacagccca	gcttggagcg	aacgacctac	accgaactga	gatacctaca	3550
3551	gcgtgagcta	tgagaaagcg	ccacgcttcc	cgaagggaga	aaggcggaca	3600
3601	ggtatccggt	aagcggcagg	gtcggaacag	gagagcgcac	gagggagctt	3650
3651	ccagggggaa	acgcctggta	tctttatagt	cctgtcgggt	ttcgccacct	3700
3701	ctgacttgag	cgtcgatttt	tgtgatgctc	gtcagggggg	cggagcctat	3750
3751	ggaaaaacgc	cagcaacgcg	gcctttttac	ggttcctggc	cttttgctgg	3800
3801	ccttttgctc	acatgttctt	tcctgcgtta	tcccctgatt	ctgtggataa	3850
3851	ccgtattacc	gcctttgagt	gagctgatac	cgctcgccgc	agccgaacga	3900
3901	ccgagcgcag	cgagtcagtg	agcgaggaag	cggaagagc	3939