pEF6/V5 His TOPO

保存条件 ：低温运输，-20℃保存

保质期 ：1年

英文名 ：pEF6/V5 His TOPO

库存 ：大量

供应商 ：信裕生物

名称	编号	规格	价格	手册
pEF6/V5 His TOPO(pEF6V5HisTOPO)

质粒编号:
质粒名称: pEF6/V5 His TOPO
其他名称:
质粒大小（bp）: 5840
抗性: 氨苄青霉素（Ampicillin）
质粒类型: 哺乳细胞表达载体
是否病毒: 非病毒
启动子: EF-1a
稳转或瞬转: 瞬表达
组成型或诱导性: 组成型表达
表达水平: high
蛋白标签: His Tag (6x) ; V5 Epitope Tag
5’测序引物: T7 Forward：TAATACGACTCACTATAGGG
GenBank号:
其他属性: 5′ Sequencing 1 Primer:T7 Fwd
5′ Sequencing 1 Primer Sequence:5’d[TAATACGACTCACTATAGGG]3′
Selectable Marker:Blasticidin
Notes:

质粒图谱:

质粒序列:

1	AATATTATTG	AAGCATTTAT	CAGGGTTATT	GTCTCATGAG	CGGATACATA	50
51	TTTGAATGTA	TTTAGAAAAA	TAAACAAATA	GGGGTTCCGC	GCACATTTCC	100
101	CCGAAAAGTG	CCACCTGACG	TCGACGGATC	GGGAGATCTC	CCGATCCCCT	150
151	ATGGTCGACT	CTCAGTACAA	TCTGCTCTGA	TGCCGCATAG	TTAAGCCAGT	200
201	ATCTGCTCCC	TGCTTGTGTG	TTGGAGGTCG	CTGAGTAGTG	CGCGAGCAAA	250
251	ATTTAAGCTA	CAACAAGGCA	AGGCTTGACC	GACAATTGCA	TGAAGAATCT	300
301	GCTTAGGGTT	AGGCGTTTTG	CGCTGCTTCG	CGATGTACGG	GCCAGATATA	350
351	CGCGTTGACA	TTGATTATTG	ACTAGGCTTT	TGCAAAAAGC	TTTGCAAAGA	400
401	TGGATAAAGT	TTTAAACAGA	GAGGAATCTT	TGCAGCTAAT	GGACCTTCTA	450
451	GGTCTTGAAA	GGAGTGCCTC	GTGAGGCTCC	GGTGCCCGTC	AGTGGGCAGA	500
501	GCGCACATCG	CCCACAGTCC	CCGAGAAGTT	GGGGGGAGGG	GTCGGCAATT	550
551	GAACCGGTGC	CTAGAGAAGG	TGGCGCGGGG	TAAACTGGGA	AAGTGATGTC	600
601	GTGTACTGGC	TCCGCCTTTT	TCCCGAGGGT	GGGGGAGAAC	CGTATATAAG	650
651	TGCAGTAGTC	GCCGTGAACG	TTCTTTTTCG	CAACGGGTTT	GCCGCCAGAA	700
701	CACAGGTAAG	TGCCGTGTGT	GGTTCCCGCG	GGCCTGGCCT	CTTTACGGGT	750
751	TATGGCCCTT	GCGTGCCTTG	AATTACTTCC	ACCTGGCTGC	AGTACGTGAT	800
801	TCTTGATCCC	GAGCTTCGGG	TTGGAAGTGG	GTGGGAGAGT	TCGAGGCCTT	850
851	GCGCTTAAGG	AGCCCCTTCG	CCTCGTGCTT	GAGTTGAGGC	CTGGCCTGGG	900
901	CGCTGGGGCC	GCCGCGTGCG	AATCTGGTGG	CACCTTCGCG	CCTGTCTCGC	950
951	TGCTTTCGAT	AAGTCTCTAG	CCATTTAAAA	TTTTTGATGA	CCTGCTGCGA	1000
1001	CGCTTTTTTT	CTGGCAAGAT	AGTCTTGTAA	ATGCGGGCCA	AGATCTGCAC	1050
1051	ACTGGTATTT	CGGTTTTTGG	GGCCGCGGGC	GGCGACGGGG	CCCGTGCGTC	1100
1101	CCAGCGCACA	TGTTCGGCGA	GGCGGGGCCT	GCGAGCGCGG	CCACCGAGAA	1150
1151	TCGGACGGGG	GTAGTCTCAA	GCTGGCCGGC	CTGCTCTGGT	GCCTGGCCTC	1200
1201	GCGCCGCCGT	GTATCGCCCC	GCCCTGGGCG	GCAAGGCTGG	CCCGGTCGGC	1250
1251	ACCAGTTGCG	TGAGCGGAAA	GATGGCCGCT	TCCCGGCCCT	GCTGCAGGGA	1300
1301	GCTCAAAATG	GAGGACGCGG	CGCTCGGGAG	AGCGGGCGGG	TGAGTCACCC	1350
1351	ACACAAAGGA	AAAGGGCCTT	TCCGTCCTCA	GCCGTCGCTT	CATGTGACTC	1400
1401	CACGGAGTAC	CGGGCGCCGT	CCAGGCACCT	CGATTAGTTC	TCGAGCTTTT	1450
1451	GGAGTACGTC	GTCTTTAGGT	TGGGGGGAGG	GGTTTTATGC	GATGGAGTTT	1500
1501	CCCCACACTG	AGTGGGTGGA	GACTGAAGTT	AGGCCAGCTT	GGCACTTGAT	1550
1551	GTAATTCTCC	TTGGAATTTG	CCCTTTTTGA	GTTTGGATCT	TGGTTCATTC	1600
1601	TCAAGCCTCA	GACAGTGGTT	CAAAGTTTTT	TTCTTCCATT	TCAGGTGTCG	1650
1651	TGAGGAATTA	GCTTGGTACT	AATACGACTC	ACTATAGGGA	GACCCAAGCT	1700
1701	GGCTAGGTAA	GCTTGGTACC	GAGCTCGGAT	CCACTAGTCC	AGTGTGGTGG	1750
1751	AATTGCCCTT	AGGGCAATTC	TGCAGATATC	CAGCACAGTG	GCGGCCGCTC	1800
1801	GAGTCTAGAG	GGCCCGCGGT	TCGAAGGTAA	GCCTATCCCT	AACCCTCTCC	1850
1851	TCGGTCTCGA	TTCTACGCGT	ACCGGTCATC	ATCACCATCA	CCATTGAGTT	1900
1901	TAAACCCGCT	GATCAGCCTC	GACTGTGCCT	TCTAGTTGCC	AGCCATCTGT	1950
1951	TGTTTGCCCC	TCCCCCGTGC	CTTCCTTGAC	CCTGGAAGGT	GCCACTCCCA	2000
2001	CTGTCCTTTC	CTAATAAAAT	GAGGAAATTG	CATCGCATTG	TCTGAGTAGG	2050
2051	TGTCATTCTA	TTCTGGGGGG	TGGGGTGGGG	CAGGACAGCA	AGGGGGAGGA	2100
2101	TTGGGAAGAC	AATAGCAGGC	ATGCTGGGGA	TGCGGTGGGC	TCTATGGCTT	2150
2151	CTGAGGCGGA	AAGAACCAGC	TGGGGCTCTA	GGGGGTATCC	CCACGCGCCC	2200
2201	TGTAGCGGCG	CATTAAGCGC	GGCGGGTGTG	GTGGTTACGC	GCAGCGTGAC	2250
2251	CGCTACACTT	GCCAGCGCCC	TAGCGCCCGC	TCCTTTCGCT	TTCTTCCCTT	2300
2301	CCTTTCTCGC	CACGTTCGCC	GGCTTTCCCC	GTCAAGCTCT	AAATCGGGGC	2350
2351	ATCCCTTTAG	GGTTCCGATT	TAGTGCTTTA	CGGCACCTCG	ACCCCAAAAA	2400
2401	ACTTGATTAG	GGTGATGGTT	CACGTAGTGG	GCCATCGCCC	TGATAGACGG	2450
2451	TTTTTCGCCC	TTTGACGTTG	GAGTCCACGT	TCTTTAATAG	TGGACTCTTG	2500
2501	TTCCAAACTG	GAACAACACT	CAACCCTATC	TCGGTCTATT	CTTTTGATTT	2550
2551	ATAAGGGATT	TTGGGGATTT	CGGCCTATTG	GTTAAAAAAT	GAGCTGATTT	2600
2601	AACAAAAATT	TAACGCGAAT	TAATTCTGTG	GAATGTGTGT	CAGTTAGGGT	2650
2651	GTGGAAAGTC	CCCAGGCTCC	CCAGGCAGGC	AGAAGTATGC	AAAGCATGCA	2700
2701	TCTCAATTAG	TCAGCAACCA	GGTGTGGAAA	GTCCCCAGGC	TCCCCAGCAG	2750
2751	GCAGAAGTAT	GCAAAGCATG	CATCTCAATT	AGTCAGCAAC	CATAGTCCCG	2800
2801	CCCCTAACTC	CGCCCATCCC	GCCCCTAACT	CCGCCCAGTT	CCGCCCATTC	2850
2851	TCCGCCCCAT	GGCTGACTAA	TTTTTTTTAT	TTATGCAGAG	GCCGAGGCCG	2900
2901	CCTCTGCCTC	TGAGCTATTC	CAGAAGTAGT	GAGGAGGCTT	TTTTGGAGGC	2950
2951	CTAGGCTTTT	GCAAAAAGCT	CCCGGGAGCT	TGTATATCCA	TTTTCGGATC	3000
3001	TGATCAGCAC	GTGTTGACAA	TTAATCATCG	GCATAGTATA	TCGGCATAGT	3050
3051	ATAATACGAC	AAGGTGAGGA	ACTAAACCAT	GGCCAAGCCT	TTGTCTCAAG	3100
3101	AAGAATCCAC	CCTCATTGAA	AGAGCAACGG	CTACAATCAA	CAGCATCCCC	3150
3151	ATCTCTGAAG	ACTACAGCGT	CGCCAGCGCA	GCTCTCTCTA	GCGACGGCCG	3200
3201	CATCTTCACT	GGTGTCAATG	TATATCATTT	TACTGGGGGA	CCTTGTGCAG	3250
3251	AACTCGTGGT	GCTGGGCACT	GCTGCTGCTG	CGGCAGCTGG	CAACCTGACT	3300
3301	TGTATCGTCG	CGATCGGAAA	TGAGAACAGG	GGCATCTTGA	GCCCCTGCGG	3350
3351	ACGGTGTCGA	CAGGTGCTTC	TCGATCTGCA	TCCTGGGATC	AAAGCGATAG	3400
3401	TGAAGGACAG	TGATGGACAG	CCGACGGCAG	TTGGGATTCG	TGAATTGCTG	3450
3451	CCCTCTGGTT	ATGTGTGGGA	GGGCTAAGCA	CTTCGTGGCC	GAGGAGCAGG	3500
3501	ACTGACACGT	GCTACGAGAT	TTCGATTCCA	CCGCCGCCTT	CTATGAAAGG	3550
3551	TTGGGCTTCG	GAATCGTTTT	CCGGGACGCC	GGCTGGATGA	TCCTCCAGCG	3600
3601	CGGGGATCTC	ATGCTGGAGT	TCTTCGCCCA	CCCCAACTTG	TTTATTGCAG	3650
3651	CTTATAATGG	TTACAAATAA	AGCAATAGCA	TCACAAATTT	CACAAATAAA	3700
3701	GCATTTTTTT	CACTGCATTC	TAGTTGTGGT	TTGTCCAAAC	TCATCAATGT	3750
3751	ATCTTATCAT	GTCTGTATAC	CGTCGACCTC	TAGCTAGAGC	TTGGCGTAAT	3800
3801	CATGGTCATA	GCTGTTTCCT	GTGTGAAATT	GTTATCCGCT	CACAATTCCA	3850
3851	CACAACATAC	GAGCCGGAAG	CATAAAGTGT	AAAGCCTGGG	GTGCCTAATG	3900
3901	AGTGAGCTAA	CTCACATTAA	TTGCGTTGCG	CTCACTGCCC	GCTTTCCAGT	3950
3951	CGGGAAACCT	GTCGTGCCAG	CTGCATTAAT	GAATCGGCCA	ACGCGCGGGG	4000
4001	AGAGGCGGTT	TGCGTATTGG	GCGCTCTTCC	GCTTCCTCGC	TCACTGACTC	4050
4051	GCTGCGCTCG	GTCGTTCGGC	TGCGGCGAGC	GGTATCAGCT	CACTCAAAGG	4100
4101	CGGTAATACG	GTTATCCACA	GAATCAGGGG	ATAACGCAGG	AAAGAACATG	4150
4151	TGAGCAAAAG	GCCAGCAAAA	GGCCAGGAAC	CGTAAAAAGG	CCGCGTTGCT	4200
4201	GGCGTTTTTC	CATAGGCTCC	GCCCCCCTGA	CGAGCATCAC	AAAAATCGAC	4250
4251	GCTCAAGTCA	GAGGTGGCGA	AACCCGACAG	GACTATAAAG	ATACCAGGCG	4300
4301	TTTCCCCCTG	GAAGCTCCCT	CGTGCGCTCT	CCTGTTCCGA	CCCTGCCGCT	4350
4351	TACCGGATAC	CTGTCCGCCT	TTCTCCCTTC	GGGAAGCGTG	GCGCTTTCTC	4400
4401	AATGCTCACG	CTGTAGGTAT	CTCAGTTCGG	TGTAGGTCGT	TCGCTCCAAG	4450
4451	CTGGGCTGTG	TGCACGAACC	CCCCGTTCAG	CCCGACCGCT	GCGCCTTATC	4500
4501	CGGTAACTAT	CGTCTTGAGT	CCAACCCGGT	AAGACACGAC	TTATCGCCAC	4550
4551	TGGCAGCAGC	CACTGGTAAC	AGGATTAGCA	GAGCGAGGTA	TGTAGGCGGT	4600
4601	GCTACAGAGT	TCTTGAAGTG	GTGGCCTAAC	TACGGCTACA	CTAGAAGGAC	4650
4651	AGTATTTGGT	ATCTGCGCTC	TGCTGAAGCC	AGTTACCTTC	GGAAAAAGAG	4700
4701	TTGGTAGCTC	TTGATCCGGC	AAACAAACCA	CCGCTGGTAG	CGGTGGTTTT	4750
4751	TTTGTTTGCA	AGCAGCAGAT	TACGCGCAGA	AAAAAAGGAT	CTCAAGAAGA	4800
4801	TCCTTTGATC	TTTTCTACGG	GGTCTGACGC	TCAGTGGAAC	GAAAACTCAC	4850
4851	GTTAAGGGAT	TTTGGTCATG	AGATTATCAA	AAAGGATCTT	CACCTAGATC	4900
4901	CTTTTAAATT	AAAAATGAAG	TTTTAAATCA	ATCTAAAGTA	TATATGAGTA	4950
4951	AACTTGGTCT	GACAGTTACC	AATGCTTAAT	CAGTGAGGCA	CCTATCTCAG	5000
5001	CGATCTGTCT	ATTTCGTTCA	TCCATAGTTG	CCTGACTCCC	CGTCGTGTAG	5050
5051	ATAACTACGA	TACGGGAGGG	CTTACCATCT	GGCCCCAGTG	CTGCAATGAT	5100
5101	ACCGCGAGAC	CCACGCTCAC	CGGCTCCAGA	TTTATCAGCA	ATAAACCAGC	5150
5151	CAGCCGGAAG	GGCCGAGCGC	AGAAGTGGTC	CTGCAACTTT	ATCCGCCTCC	5200
5201	ATCCAGTCTA	TTAATTGTTG	CCGGGAAGCT	AGAGTAAGTA	GTTCGCCAGT	5250
5251	TAATAGTTTG	CGCAACGTTG	TTGCCATTGC	TACAGGCATC	GTGGTGTCAC	5300
5301	GCTCGTCGTT	TGGTATGGCT	TCATTCAGCT	CCGGTTCCCA	ACGATCAAGG	5350
5351	CGAGTTACAT	GATCCCCCAT	GTTGTGCAAA	AAAGCGGTTA	GCTCCTTCGG	5400
5401	TCCTCCGATC	GTTGTCAGAA	GTAAGTTGGC	CGCAGTGTTA	TCACTCATGG	5450
5451	TTATGGCAGC	ACTGCATAAT	TCTCTTACTG	TCATGCCATC	CGTAAGATGC	5500
5501	TTTTCTGTGA	CTGGTGAGTA	CTCAACCAAG	TCATTCTGAG	AATAGTGTAT	5550
5551	GCGGCGACCG	AGTTGCTCTT	GCCCGGCGTC	AATACGGGAT	AATACCGCGC	5600
5601	CACATAGCAG	AACTTTAAAA	GTGCTCATCA	TTGGAAAACG	TTCTTCGGGG	5650
5651	CGAAAACTCT	CAAGGATCTT	ACCGCTGTTG	AGATCCAGTT	CGATGTAACC	5700
5701	CACTCGTGCA	CCCAACTGAT	CTTCAGCATC	TTTTACTTTC	ACCAGCGTTT	5750
5751	CTGGGTGAGC	AAAAACAGGA	AGGCAAAATG	CCGCAAAAAA	GGGAATAAGG	5800
5801	GCGACACGGA	AATGTTGAAT	ACTCATACTC	TTCCTTTTTC	5840