Strep-Tactin®XT 蛋白纯化磁珠 MagStrep "type3" XT beads 5% suspension 价格

保存条件：4°C

供应商：IBA Lifesciences中国正式代理商

MagStrep “type3” XT beads是经Strep-Tactin®XT涂布的磁珠，具有以下特点:

1. 能与高量蛋白结合: 约0.85 nmol/μl beads, 即25.5 μg的30 kDa蛋白
2. 低背景: 非特异性的结合少
3. 具有弹性的洗脱方式: 可直接加入SDS gel loading buffer分析，也可以使用Biotin将目标蛋白从磁珠上洗脱
4. 与Strep-tag®及Twin-Strep-tag®的亲和力极高: 归功于磁珠上的Strep-Tactin®XT

综合以上特点，使用MagStrep “type3” XT beads可得到很好的纯化效果(见下方实验结果)。因此，对于需要高纯度蛋白的您，此磁珠是最适用于小规模、快速蛋白质纯化的工具。

使用MagStrep “type3” XT beads从原始细菌提取物中纯化GFP-Strep-tag融合蛋白

使用Agilent Bioanalyzer 2100 System分析提取出来的蛋白，发现不管是直接以Buffer BX (含Biotin)将靶蛋白洗脫下來，或是加入sample buffer煮沸来分离目标蛋白，都能得到高纯度的蛋白。

MagStrep "type3" XT磁珠及相关产品

产品		容量	货号
磁珠	MagStrep "type3" XT beads 5% suspension	2 ml	2-4090-002
相容磁座	Magnetic Separator	for 24 reaction tubes	2-1602-000
洗脱溶液	Buffer BXT; Biotin Elution Buffer for Strep-Tactin® XT; ready-to-use	250 ml	2-1041-250
其他相关试剂	His-STREPPER	0.5 ml	2-0920-005

MagStrep “type3” XT beads are magnetic beads consisting of a 6% agarose coated with the streptavidin variant Strep-Tactin^®XT and can be used for Strep-tag^®II and Twin-Strep-tag^® fusion proteins. Due to the magnetic core no centrifugation step is required since the beads can be easily and swiftly separated with a magnetic separator, reducing the risk of disturbing the pellet or loss of beads within the wash step to zero.
As with any other Strep-Tactin^®XT resins, the unspecific protein binding is extremely low, which results in an outstanding purity of the target protein. However, MagStrep “type3” XT beads provide the highest binding capacity. These characteristics make them a perfect tool for isolation of proteins or complexes via pull-downs, immunoprecipitation (IP), or co-immunoprecipitation (Co-IP) in batch format and/or high-throughput screenings.
The elution of target proteins or complexes can be triggered under mild conditions with the biotin-containing Buffer BXT or under denaturing conditions by boiling in SDS gel loading buffer. 20 μl suspension correspond to 1 μl magnetic beads.

Specifications

Bead Size:	25 μm (average)
Form:	5% suspension
Possible Application:	Protein purification of Strep-tag^®II and Twin-Strep-tag^® fusion proteins
Specificity:	Twin-Strep-tag^® and Strep-tag^®II
Strep-tag^®II Binding Capacity:	25.5 mg/ml
Support:	6% agarose, crosslinked, spherical magnetic beads