Anti-MOG Antibody价格_品牌:BOSTER-丁香通官网

克隆性：Polyclonal

适应物种：human, mouse, rat

保质期：发货周期：现货

库存：9999

供应商：博士德生物

应用范围：WB, IHC, FCM

规格：50μl/100μl/150μl

产品概况

货号	A03294
产品名称	Anti-MOG Antibody
基因名	MOG
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	28KD
免疫原	A synthetic peptide corresponding to a sequence of human Myelin oligodendrocyte glycoprotein/MOG(RVVHLYRNGKDQDGDQAPEYRGRTELLKDAIGEGK).
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Myelin oligodendrocyte glycoprotein (MOG) is a glycoprotein believed to be important in the myelination of nerves in the central nervous system (CNS). In humans this protein is encoded by the MOG gene. This gene is mapped to 6p22.1. It is speculated to serve as a necessary "adhesion molecule" to provide structural integrity to the myelin sheath and is known to develop late on the oligodendrocyte. The product of this gene is a membrane protein expressed on the oligodendrocyte cell surface and the outermost surface of myelin sheaths. Due to this localization, it is a primary target antigen involved in immune-mediated demyelination. This protein may be involved in completion and maintenance of the myelin sheath and in cell-cell communication. Alternatively spliced transcript variants encoding different isoforms have been identified.
研究类别	1. Pham-Dinh D, Della Gaspera B, Kerlero de Rosbo N, Dautigny A (September 1995). "Structure of the human myelin/oligodendrocyte glycoprotein gene and multiple alternative spliced isoforms". Genomics. 2. Pham-Dinh D, Jones EP, Pitiot G, Della Gaspera B, Daubas P, Mallet J, Le Paslier D, Fischer Lindahl K, Dautigny A (1995). 3. "Physical mapping of the human and mouse MOG gene at the distal end of the MHC class Ib region". 4. Immunogenetics. Roth MP, Malfroy L, Offer C, Sevin J, Enault G, Borot N, Pontarotti P, Coppin H (July 1995). 5. "The human myelin oligodendrocyte glycoprotein (MOG) gene: complete nucleotide sequence and structural characterization". Genomics. 6. Berger, T., Innsbruck Medical University Dept. of Neurology interviewed by S. Gillooly, Nov. 24, 2008.
Uniprot ID	MOG: Q16653
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunohistochemistry in paraffin section (IHC):	1:50-400
Flow cytometry (FCM):	1-3 μg/1x10⁶ cells
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of Myelin oligodendrocyte glycoprotein using anti-Myelin oligodendrocyte glycoprotein antibody (A03294).
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Myelin oligodendrocyte glycoprotein at approximately 26KD. The expected band size for Myelin oligodendrocyte glycoprotein is at 28KD.|Figure 2. IHC analysis of Myelin oligodendrocyte glycoprotein using anti-Myelin oligodendrocyte glycoprotein antibody (A03294).
Myelin oligodendrocyte glycoprotein was detected in paraffin-embedded section of human glioma tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis of Myelin oligodendrocyte glycoprotein using anti-Myelin oligodendrocyte glycoprotein antibody (A03294).
Myelin oligodendrocyte glycoprotein was detected in paraffin-embedded section of mouse brain tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis of Myelin oligodendrocyte glycoprotein using anti-Myelin oligodendrocyte glycoprotein antibody (A03294).
Myelin oligodendrocyte glycoprotein was detected in paraffin-embedded section of rat brain tissues.Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis of Myelin oligodendrocyte glycoprotein using anti-Myelin oligodendrocyte glycoprotein antibody (A03294).
Myelin oligodendrocyte glycoprotein was detected in paraffin-embedded section of rat brain tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 6. Flow Cytometry analysis of U251 cells using anti- Myelin oligodendrocyte glycoprotein antibody (A03294).
Overlay histogram showing U251 cells stained with A03294 (Blue line). DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]