Anti-BCL2L1 Antibody价格_品牌:BOSTER-丁香通官网

克隆性：Polyclonal

适应物种：human, mouse, rat

保质期：发货周期：现货

库存：9999

供应商：博士德生物

应用范围：WB, IHC, IHC-F, ICC, FCM

规格：50μl/100μl/150μl

产品概况

货号	A00181
产品名称	Anti-BCL2L1 Antibody
基因名	BCL2L1
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	26KD
免疫原	E.coli-derived human Bcl-XL recombinant protein (Position: M1-T219). Human Bcl-XL shares 97.9% amino acid (aa) sequence identity with both mouse and rat Bcl-XL.
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Bcl-2-like protein 1, also known as Bcl-XL, is a protein that in humans is encoded by the BCL2L1 gene. The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform (Bcl-xL) acts as an apoptotic inhibitor and the shorter form (Bcl-xS) acts as an apoptotic activator.
研究类别	1. "Entrez Gene: BCL2L1 BCL2-like 1".2. Boise LH, González-García M, Postema CE, Ding L, Lindsten T, Turka LA, Mao X, Nu ez G, Thompson CB (Aug 1993). "bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death". Cell 74(4): 597–608.
Uniprot ID	BCL2L1: Q07817
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunohistochemistry in paraffin section (IHC):	1:50-400
Immunohistochemistry in frozen section:	1:50-400
Immunocytochemistry in fixed cells:	1:50-400
Flow cytometry (FCM):	1-3 μg/1x10⁶ cells
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of Bcl-XL using anti-Bcl-XL antibody (A00181). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates,Lane 2: NIH3T3 whole cell lysates,Lane 3: HEPA1-6 whole cell lysates,Lane 4: SGC7901 whole cell lysates,Lane 5: HEPG2 whole cell lysates.probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Bcl-XL at approximately 30KD. The expected band size for Bcl-XL is at 26KD.|Figure 2. IHC analysis of Bcl-XL using anti-Bcl-XL antibody (A00181). detected in paraffin-embedded section of human tonsil tissues.. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis of Bcl-XL using anti-Bcl-XL antibody (A00181). detected in paraffin-embedded section of human lung cancer tissues. . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis of Bcl-XL using anti-Bcl-XL antibody (A00181).detected in paraffin-embedded section of human mammary cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 5. Flow Cytometry analysis of PC-3 cells using anti-Bcl-XL antibody (A00181).Overlay histogram showing PC-3 cells stained with A00181 (Blue line).. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]