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保存条件 ：负20度
英文名 ：Recombinant Dickkopf Related Protein 3 (DKK3)
库存 ：大量
供应商 ：柯雷生物
[ PROPERTIES ]
Residues: Pro41~Ile348
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: B1H219
Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 4.5
Predicted Molecular Mass: 38.2kDa
Accurate Molecular Mass: 46kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
PQEEATLNEM FREVEELMED TQHKLRSAVE EMEAEEAAAR TSSEVTLSSL PANYHNETNT
ETRMENNTAH VHREVHKITN NQSGQTVFSE TVITSVEDGE GKKSHECIID EDCGPTRYCQ
FSSF KYT CQP CR DQ QM L C T R DSE CC GD Q L C AWG HC TQKAT KGS N GTI CD N
QRDCQPGLCC AFQRGLLFPV CTPLPVEGEL CHDPTSQMLD LITWELEPEG ALDRCPCASG
LLCQPHSHSL VYMCKPAFVG SHDHNEESQL PREALDDYED VGFIGEVRQE LEDLERSLAQ
EMAFEEATPV DSLGGEKI
上海柯雷生物科技有限公司
实名认证
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入驻年限：8年
